Real-time extended depth of field microscopy
Real-time extended depth of field microscopy
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DOI：
10.1364/oe.16.021843
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发表时间：
2008-12-22
期刊：
影响因子：
3.8
通讯作者：
Wilson, Tony
中科院分区：
文献类型：
--
作者：
Botcherby, Edward J.;Booth, Martin J.;Wilson, Tony
我们描述了一种光学显微镜系统，其焦点设置可以迅速改变，而无需移动物镜透镜或样品。使用该系统，可以从宽范围的焦点设置获取衍射极限图像，而不会引入降低图像质量的光学像差。我们结合联合收割机这个系统与一个真实的时间Nipkow光盘为基础的共聚焦显微镜，以便允许扩展的景深图像直接在一个单一的CCD相机帧的收购。我们还演示了一个简单的修改，使扩展的景深图像从不同的视角，其中的角度可以在一个连续的范围内由用户实时改变。(C)2008年美国光学学会
We describe an optical microscope system whose focal setting can be changed quickly without moving the objective lens or specimen. Using this system, diffraction limited images can be acquired from a wide range of focal settings without introducing optical aberrations that degrade image quality. We combine this system with a real time Nipkow disc based confocal microscope so as to permit the acquisition of extended depth of field images directly in a single frame of the CCD camera. We also demonstrate a simple modification that enables extended depth of field images to be acquired from different angles of perspective, where the angle can be changed over a continuous range by the user in real-time. (C) 2008 Optical Society of America