Endotoxin adsorbent using dimethylamine ligands.
Endotoxin adsorbent using dimethylamine ligands.
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DOI：
10.1016/j.biomaterials.2004.07.027
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发表时间：
2005-05
期刊：
影响因子：
14
通讯作者：
Zhi Yuan;Mei Yu;Jihong Li;Guanghui Hou;Huiyan Wang
中科院分区：
文献类型：
--
作者：
Zhi Yuan;Mei Yu;Jihong Li;Guanghui Hou;Huiyan Wang
研究了各种吸附剂去除蛋白质溶液中的内毒素。认为静电相互作用和疏水分子间相互作用是内毒素吸附的主要相互作用。本文通过在聚甲基丙烯酸甲酯上偶联二甲胺配体，制备了一系列新型分子识别吸附剂，用于去除内毒素。我们发现，在配体的β-位点存在羟基时，其对内毒素的吸附能力增加了近8倍。计算机模拟表明，β-位点的羟基可以与内毒素形成H键，形成一个八原子环。吸附剂中的间隔链与内毒素中的长烷基链位于八原子环的同侧。在这种情况下，静电相互作用、氢键、八原子环的协同效应和疏水分子间相互作用同时发生。内毒素与吸附剂结合紧密，有效提高了吸附量。
Various adsorbents have been investigated for removing endotoxin from protein solutions. It is believed that electrostatic interaction and hydrophobic intermolecular interaction are the main interactions in adsorption of endotoxin. In this work, a series of novel molecular recognition adsorbents for removal of endotoxin with dimethylamine ligand were prepared by coupling ligands on polymethyl methacrylate. We found that its adsorption capacity of endotoxin increased almost 8 times in the presence of a hydroxyl group at β-site of ligand. The computer simulation showed that the hydroxyl group at β-site could form H bond with endotoxin, as a result an octatomic ring was formed. The spacer in adsorbent and the long alkyl chain in endotoxin were located at the same side of the octatomic ring. In this situation, electrostatic interaction, H bond, cooperative effect of octatomic ring and hydrophobic intermolecular interaction effected simultaneously. The combination of endotoxin with adsorbent was tight and adsorption capacity was effectually increased.