Purinergic receptors are part of a signalling system for proliferation and differentiation in distinct cell lineages in human anagen hair follicles
Purinergic receptors are part of a signalling system for proliferation and differentiation in distinct cell lineages in human anagen hair follicles
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DOI：
10.1007/s11302-008-9108-0
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发表时间：
2008-12-01
影响因子：
3.5
通讯作者：
Burnstock, Geoffrey
中科院分区：
文献类型：
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作者：
Greig, Aina V. H.;Linge, Claire;Burnstock, Geoffrey
我们研究了成人毛发生长初期毛囊中 P2X(5)、P2X(7)、P2Y(1) 和 P2Y(2) 受体亚型的表达及其与增殖标记物[增殖细胞核抗原 (PCNA) 和 Ki-67]、角质形成细胞分化（外皮蛋白）和细胞凋亡（anticaspase-3）的关系。使用免疫组织化学，我们发现P2X（5），P2Y（1）和P2Y（2）受体在生长期毛囊的空间不同区域中表达：P2Y（1）受体在外根鞘和球部，P2X（5）受体在内外根鞘和髓质中，P2Y（2）受体在皮层/髓质边缘的活细胞中。 P2X(7) 受体不表达。共定位实验表明这些受体具有不同的功能作用：P2Y（1）受体与球和外根鞘角质形成细胞增殖相关，P2X（5）受体与髓质和内根鞘细胞的分化相关，P2Y（2）受体与皮质/髓质中有助于毛干形成的早期分化细胞相关。讨论了嘌呤能激动剂和拮抗剂控制毛发生长的治疗潜力。
We investigated the expression of P2X(5), P2X(7), P2Y(1) and P2Y(2) receptor subtypes in adult human anagen hair follicles and in relation to markers of proliferation [proliferating cell nuclear antigen (PCNA) and Ki-67], keratinocyte differentiation (involucrin) and apoptosis (anticaspase-3). Using immunohistochemistry, we showed that P2X(5), P2Y(1) and P2Y(2) receptors were expressed in spatially distinct zones of the anagen hair follicle: P2Y(1) receptors in the outer root sheath and bulb, P2X(5) receptors in the inner and outer root sheaths and medulla and P2Y(2) receptors in living cells at the edge of the cortex/medulla. P2X(7) receptors were not expressed. Colocalisation experiments suggested different functional roles for these receptors: P2Y(1) receptors were associated with bulb and outer root sheath keratinocyte proliferation, P2X(5) receptors were associated with differentiation of cells of the medulla and inner root sheaths and P2Y(2) receptors were associated with early differentiated cells in the cortex/medulla that contribute to the formation of the hair shaft. The therapeutic potential of purinergic agonists and antagonists for controlling hair growth is discussed.