RNA-POLYMERASE OF INFLUENZA-VIRUS .5. ACTIVATION OF INFLUENZA VIRUS-ASSOCIATED RNA-POLYMERASE BY CAP-1 STRUCTURE (M7GPPPNM)
RNA-POLYMERASE OF INFLUENZA-VIRUS .5. ACTIVATION OF INFLUENZA VIRUS-ASSOCIATED RNA-POLYMERASE BY CAP-1 STRUCTURE (M7GPPPNM)
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DOI：
10.1093/oxfordjournals.jbchem.a135101
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
MIURA, KI
中科院分区：
文献类型：
--
作者：
KAWAKAMI, K;MIZUMOTO, K;MIURA, KI
在通过加帽RNA的内切核酸裂解产生用于转录起始的引物期间，流感病毒相关的RNA依赖性RNA聚合酶识别加帽RNA的3个信号，即，5“末端的cap-1结构、切割位点的RNA序列和这2个信号之间的距离（Plotch，S. J.，Bouloy，M.，和Krug，R.M.（1979），Kawakami，K.，Mizumoto，K.，和Ishihama，A.（1983））。游离帽-1结构，即，不与多核苷酸结合的m7 GpppNm [7-甲基鸟苷（8“）三磷酸（5”）-2“-O-甲基核苷]也刺激RNA合成，而不管二核苷酸引物的存在与否。由于m7 GpppNm和ApG [腺苷酰-3“，5”-鸟苷]的刺激是相加的，并且游离cap-1结构不掺入产物RNA的5“末端，所以cap-1结构通过变构调节而不是通过引发转录起始来刺激RNA聚合酶。
During the generation of primer for transcription initiation by endonucleolytic cleavage of capped RNA, the influenza virus-associated RNA-dependent RNA polymerase recognizes 3 signals of capped RNA, i.e., cap-1 structure at 5'' termini, RNA sequences at the cleavage sites and distance between these 2 signals (Plotch, S.J., Bouloy, M., and Krug, R.M. (1979), Kawakami, K., Mizumoto, K., and Ishihama, A. (1983)). The free cap-1 structure, i.e., m7GpppNm [7-methylguanosine (8'')triphosphate (5'')-2''-O-methylnucleoside] not associated with polynucleotides, stimulates the RNA synthesis as well, irrespective of the presence or absence of dinucleotide primers. Since the stimulation by m7GpppNm and ApG [adenylyl-3'',5''-guanosine] is additive and the free cap-1 structure is not incorporated into 5'' termini of product RNA, the cap-1 structure stimulates the RNA polymerase by allosteric modulation rather than by priming the transcription initiation.