Direct-current electrical field guides neuronal stem/progenitor cell migration
Direct-current electrical field guides neuronal stem/progenitor cell migration
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DOI：
10.1634/stemcells.2007-1022
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发表时间：
2008-08-01
期刊：
影响因子：
5.2
通讯作者：
Wan, Qi
中科院分区：
文献类型：
--
作者：
Li, Lei;El-Hayek, Youssef H.;Wan, Qi
直流电场（EF）促进神经生长和轴突再生。我们在这里报告，在生理强度，EFs引导神经元干/祖细胞（NSPCs）向阴极迁移。F，F-指导的NSPC迁移需要Nuriethyl-D-天冬氨酸受体（NMDAR）的活化，这导致Rho GTdR Rac相关信号与膜NMDAR和细胞内肌动蛋白细胞骨架的物理缔合增加。因此，本研究确定EF作为控制NSPC迁移的定向引导线索，并揭示NMDAR/Racl/ actin信号转导通路在介导EF诱导的NSPC迁移中的作用。这些结果表明，作为一种安全的物理方法在临床应用中，EFs可能被开发作为一个实用的治疗策略，脑修复，通过引导NSPC迁移到受损的脑区域，以取代细胞损失。
Direct-current electrical fields (EFs) promote nerve growth and axon regeneration. We report here that at physiological strengths, EFs guide the migration of neuronal stem/progenitor cells (NSPCs) toward the cathode. F,F-directed NSPC migration requires activation of Nuriethyl-D-aspartate receptors (NMDARs), which leads to an increased physical association of Rho GTPase Ractassociated signals to the membrane NMDARs and the intracellular actin cytoskeleton. Thus, this study iden- tifies the EF as a directional guidance cue in controlling NSPC migration and reveals a role of the NMDAR/Racl/ actin signal transduction pathway in mediating EF-induced NSPC migration. These results suggest that as a safe physical approach in clinical application, EFs may be developed as a practical therapeutic strategy for brain repair by directing NSPC migration to the injured brain regions to replace cell loss.