Markedly increased amounts of messenger RNAs for vascular endothelial growth factor and placenta growth factor in renal cell carcinoma associated with angiogenesis.
Markedly increased amounts of messenger RNAs for vascular endothelial growth factor and placenta growth factor in renal cell carcinoma associated with angiogenesis.
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发表时间：
1994-08
期刊：
影响因子：
11.2
通讯作者：
Atsushi Takahashi;H. Sasaki;Sun Jin Kim;K. Tobisu;T. Kakizoe;T. Tsukamoto;Y. Kumamoto;Takashi Sugimura;M. Terada
中科院分区：
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作者：
Atsushi Takahashi;H. Sasaki;Sun Jin Kim;K. Tobisu;T. Kakizoe;T. Tsukamoto;Y. Kumamoto;Takashi Sugimura;M. Terada
在29例肾细胞癌组织和邻近的正常肾组织以及4个人肾细胞癌细胞系中检测了血管内皮生长因子（VEGF）及其相关蛋白胎盘生长因子（PIGF） mrna的表达。Northern blot分析显示，27例高血管性肾细胞癌组织中有26例（96%）的VEGF mRNA水平较邻近正常肾组织显著升高（3-13倍）。即使是小肿瘤，只要是血管增生，也会过度表达VEGF mRNA。我们还证实，PIGF mRNA在23例高血管肾细胞癌组织中的21例（91%）中表达，但在邻近的正常肾组织中未检测到。两种低血管癌组织既没有过表达VEGF mRNA，也没有过表达PIGF mRNA。4种人肾细胞癌细胞系中均检测到VEGF mRNA，未检测到PIGF mRNA。尽管我们之前的研究表明肾癌患者血清中碱性成纤维细胞生长因子蛋白升高（K. Fujimoto et al., Biochem），但肿瘤组织和正常肾脏组织中碱性成纤维细胞生长因子mRNA的水平没有差异。Biophys。Commun >,中文信息学报，18(3):386-392,1991)。综上所述，这些结果表明VEGF、PIGF和碱性成纤维细胞生长因子在体内共同促进肾细胞癌血管生成。
The presence of mRNAs for vascular endothelial growth factor (VEGF) and a VEGF-related protein, placenta growth factor (PIGF) was examined in 29 cases of renal cell carcinoma tissues and adjacent normal kidney tissues and in 4 human renal cell carcinoma cell lines. Northern blot analysis showed that 26 of 27 hypervascular renal cell carcinoma tissues (96%) exhibited a markedly elevated level (3-13 fold) of VEGF mRNA compared to the adjacent normal kidney tissues. Even tumors of small size, whenever they were hypervascular, overexpressed VEGF mRNA. We also demonstrated that mRNA for PIGF was expressed in 21 of 23 hypervascular renal cell carcinoma tissues (91%) but was not detected in the adjacent normal kidney tissues. Two hypovascular carcinoma tissues neither overexpressed VEGF mRNA nor had PIGF mRNA. VEGF mRNA was detected in four human renal cell carcinoma cell lines, while PIGF mRNA was not. There was no difference in the level of basic fibroblast growth factor mRNA between tumor tissues and normal kidney tissue, although our previous study demonstrated elevated basic fibroblast growth factor protein in the serum of renal cell carcinoma patients (K. Fujimoto et al., Biochem. Biophys. Res. Commun., 180: 386-392, 1991). Taken together, these results suggest that VEGF, PIGF, and basic fibroblast growth factor are cooperatively working to increase the angiogenesis in renal cell carcinoma in vivo.