Isolation and characterization of an orthoreovirus from Indonesian fruit bats
Isolation and characterization of an orthoreovirus from Indonesian fruit bats
复制标题
印度尼西亚果蝠正呼肠孤病毒的分离和鉴定
DOI：
10.1016/j.virol.2022.08.003
复制
发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Sasaki Michihito
中科院分区：
文献类型：
--
作者：
Intaruck Kittiya;Itakura Yukari;Kishimoto Mai;Chambaro Herman M.;Setiyono Agus;Handharyani Ekowati;Uemura Kentaro;Harima Hayato;Taniguchi Satoshi;Saijo Masayuki;Kimura Takashi;Orba Yasuko;Sawa Hirofumi;Sasaki Michihito
纳尔逊湾正射病毒(NBV)是一种新出现的蝙蝠传播病毒，可引起人类零星的呼吸道感染。在过去的二十年里，从人类和各种蝙蝠物种中分离到了几种与NBV基因相关的毒株，主要是在东南亚(SEA)，这表明NBV物种在该地区的高流行率。本研究从印尼果蝠粪便中分离到一株属于NBV属的正病毒(OroreVirus，ORV)，暂定名为Pguyaman OroreVirus(PgORV)。血清学研究表明，81.2%(108/133)的印度尼西亚果蝠血清中存在PgORV中和抗体。PgORV的全基因组测序和系统发育分析表明，过去曾与其他海洋分离的NBV毒株发生过重组，表明NBV在该地区蝙蝠中的传播和传播。实验小鼠鼻内接种PgORV可引起严重肺炎。我们的研究描述了PgORV独特的遗传背景，并强调了印度尼西亚PgORV相关疾病的潜在风险。
Nelson Bay orthoreovirus (NBV) is an emerging bat-borne virus and causes respiratory tract infections in humans sporadically. Over the last two decades, several strains genetically related to NBV were isolated from humans and various bat species, predominantly in Southeast Asia (SEA), suggesting a high prevalence of the NBV species in this region. In this study, an orthoreovirus (ORV) belonging to the NBV species was isolated from Indonesian fruit bats' feces, tentatively named Paguyaman orthoreovirus (PgORV). Serological studies revealed that 81.2% (108/133) of Indonesian fruit bats sera had neutralizing antibodies against PgORV. Whole-genome sequencing and phylogenetic analysis of PgORV suggested the occurrence of past reassortments with other NBV strains isolated in SEA, indicating the dispersal and circulation of NBV species among bats in this region. Intranasal PgORV inoculation of laboratory mice caused severe pneumonia. Our study characterized PgORV's unique genetic background and highlighted the potential risk of PgORV-related diseases in Indonesia.