The carboxy-terminus of protein 4.1R resembles beta-tubulin
The carboxy-terminus of protein 4.1R resembles beta-tubulin
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DOI：
10.1006/cbir.2002.0866
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发表时间：
2002-01-01
影响因子：
3.9
通讯作者：
Merdes, A
中科院分区：
文献类型：
--
作者：
Fant, X;Merdes, A
4.1 r蛋白是一个更大的4.1蛋白家族的同工异构体。它被认为是质膜骨架的一个组成部分，但它也存在于间期和有丝分裂的中心体中。为了研究4.1R蛋白羧基末端区域的特性，我们构建了针对4.1R最后14个氨基酸的肽的抗体。这些抗体与β -微管蛋白表位发生交叉反应，并通过免疫荧光染色微管网络。此外，羧基末端4.1R肽序列与微管蛋白的序列比较发现，与β -微管蛋白螺旋5末端的一个区域同源，而与α -微管蛋白不同源。讨论了4.1R羧基端在调控微管网络形成中的潜在作用。(C) 2002 Elsevier Science Ltd.版权所有。
The protein 4.1R is an isoform of a larger family of 4.1 proteins. It is known as a component of the plasma membrane skeleton, but it is also found at the centrosomes in interphase and mitosis. To investigate the properties of the carboxy terminal region of protein 4.1R, we raised antibodies against a peptide representing the last 14 amino acids of 4.1R. These antibodies crossreact with an epitope in beta-tubulin and stain the microtubule network by immunofluorescence. Furthermore, sequence comparison of the carboxy terminal 4.1R peptide sequence with tubulin reveals homology with a region at the end of helix 5 in beta-tubulin, but not alpha-tubulin. A potential function of the 4.1R carboxy terminus in regulating the formation of microtubule networks is discussed. (C) 2002 Elsevier Science Ltd. All rights reserved.