Biological effects of agglomerated multi-walled carbon nanotubes
Biological effects of agglomerated multi-walled carbon nanotubes
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团聚多壁碳纳米管的生物学效应
DOI：
10.1016/j.colsurfb.2016.02.032
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发表时间：
2016
影响因子：
5.8
通讯作者：
Wang Haifang
中科院分区：
文献类型：
--
作者：
Song Zheng-Mei;Wang Lin;Chen Ni;Cao Aoneng;Liu Yuanfang;Wang Haifang
纳米材料的物理化学性质在决定其生物效应方面起着至关重要的作用。纳米材料在各种体系中的团聚是一种普遍现象，但由于其复杂性，团聚对纳米材料生物学效应的影响还没有得到很好的研究。在此，我们通过使用Ca 2+制备了不同尺寸的氧化多壁碳纳米管（O-MWCNTs）团聚体，并研究了它们在HeLa细胞中的细胞摄取和细胞毒性。我们发现，O-MWCNTs团聚体的性质改变可以通过Ca 2+的量来控制和调节。团聚显着促进细胞摄取的O-MWCNTs在初始接触阶段，由于团聚体容易与细胞接触。但当O-MWCNTs浓度小于150 μg/mL时，团聚并不引起明显的细胞毒性。通过细胞增殖、细胞膜完整性、细胞凋亡和活性氧产生来检测。这项研究表明，纳米材料的生物学行为可以通过它们的团聚状态来改变。
The physicochemical properties of nanomaterials play crucial roles in determining their biological effects. Agglomeration of nanomaterials in various systems is a common phenomenon, however, how agglomeration affects the biological consequence of nanomaterials has not been well investigated because of its complexity. Herein, we prepared variable sized agglomerates of oxidized multi-walled carbon nanotubes (O-MWCNTs) by using Ca2+and studied their cellular uptake and cytotoxicity in HeLa cells. We found the altered property of O-MWCNTs agglomerates could be controlled and adjusted by the amount of Ca2+. Agglomeration remarkably facilitated the cellular uptake of O-MWCNTs at the initial contact stage, due to the easy contact of agglomerates with cells. But agglomeration did not induce evident cytotoxicity when the concentration of O-MWCNTs was less than 150 μg/mL. That was assayed by cell proliferation, membrane integrity, apoptosis and ROS generation. This study suggests us that the biological behaviors of nanomaterials could be altered by their states of agglomeration.