Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone-nanohydroxyapatite composite.
Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone-nanohydroxyapatite composite.
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DOI：
10.2147/ijn.s75557
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发表时间：
2015
影响因子：
8
通讯作者：
Wei S
中科院分区：
文献类型：
--
作者：
Deng Y;Liu X;Xu A;Wang L;Luo Z;Zheng Y;Deng F;Wei J;Tang Z;Wei S
碳纤维增强聚醚醚酮(CFRPEEK)作为美国食品和药物管理局(FDA)批准的可植入材料，具有与皮质骨相似的可调弹性模量，是替代外科金属植入物的首选材料。然而，CFRPEEK的生物惰性和较差的成骨性能限制了其作为骨科/牙科植入物的临床应用。本研究成功地制备了表面粗糙度可变的CFRPEEK-纳米羟基磷灰石三元复合材料(PEEK/n-HA/CF)。研究了表面粗糙度对成骨样MG-63细胞的体外细胞反应(贴壁、增殖、凋亡和分化)以及体内骨结合的影响。结果表明，随着复合材料表面粗糙度的增加，复合材料表面的亲水性和表面钙离子含量显著提高。在细胞培养试验中，结果表明细胞的增殖率和细胞的成骨分化程度是表面粗糙度大小的函数。与其他组相比，表面粗糙度适中的复合材料显著增加了细胞的附着/增殖，促进了碱性磷酸酶(ALP)活性的产生和钙结节的形成。更重要的是，合适表面粗糙度的PEEK/n-HA/CF种植体在动物实验中表现出显著的生物活性和体内骨整合。这些发现将为设计具有最佳粗糙度的CFRPEEK植入物提供关键指导，以调节细胞行为，并提高生物相容性和骨整合。同时，具有最佳表面粗糙度的PEEK/n-HA/CF三元复合材料有望作为生物活性生物材料应用于骨移植和组织工程领域。
As United States Food and Drug Administration-approved implantable material, carbon fiber-reinforced polyetheretherketone (CFRPEEK) possesses an adjustable elastic modulus similar to cortical bone and is a prime candidate to replace surgical metallic implants. The bioinertness and inferior osteogenic properties of CFRPEEK, however, limit its clinical application as orthopedic/dental implants. In this study, CFRPEEK–nanohydroxyapatite ternary composites (PEEK/n-HA/CF) with variable surface roughness have been successfully fabricated. The effect of surface roughness on their in vitro cellular responses of osteoblast-like MG-63 cells (attachment, proliferation, apoptosis, and differentiation) and in vivo osseointegration is evaluated. The results show that the hydrophilicity and the amount of Ca ions on the surface are significantly improved as the surface roughness of composite increases. In cell culture tests, the results reveal that the cell proliferation rate and the extent of osteogenic differentiation of cells are a function of the size of surface roughness. The composite with moderate surface roughness significantly increases cell attachment/proliferation and promotes the production of alkaline phosphatase (ALP) activity and calcium nodule formation compared with the other groups. More importantly, the PEEK/n-HA/CF implant with appropriate surface roughness exhibits remarkably enhanced bioactivity and osseointegration in vivo in the animal experiment. These findings will provide critical guidance for the design of CFRPEEK-based implants with optimal roughness to regulate cellular behaviors, and to enhance biocompability and osseointegration. Meanwhile, the PEEK/n-HA/CF ternary composite with optimal surface roughness might hold great potential as bioactive biomaterial for bone grafting and tissue engineering applications.