Using isotope tracers to study metabolism: application in mouse models
Using isotope tracers to study metabolism: application in mouse models
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DOI：
10.1016/j.ymben.2003.09.003
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发表时间：
2004-01-01
影响因子：
8.4
通讯作者：
Previs, SF
中科院分区：
文献类型：
--
作者：
McCabe, BJ;Previs, SF
讨论了同位素示踪技术在研究小鼠代谢中的应用。为了使读者熟悉，概述了有关示踪剂方法使用的一些基本原则。重点是展示研究人员如何使用同位素示踪剂来研究体内碳水化合物，脂肪和/或蛋白质周转的调节。最后，使用标记水的一些优点（即，（H2O）-H-2和/或（H2O）-O-18）来追踪生物过程的动力学。本报告提供的背景应有助于工程师设计研究，以增强我们对代谢改变的条件的理解（例如，糖尿病、癌症恶病质、不能茁壮成长和在零重力下旅行）。(C)2003年爱思唯尔公司All rights reserved.
The application of isotope tracers for investigating metabolism in mice is discussed. To familiarize the reader, some basic principles regarding the use of tracer methods are outlined. Emphasis is placed on showing how investigators are using isotope tracers to study the regulation of carbohydrate, fat and/or protein turnover in vivo. Finally, some of the advantages of using labeled water (i.e., (H2O)-H-2 and/or (H2O)-O-18) to trace the kinetics of biological processes ate considered. The background provided in this report should assist engineers in designing studies that enhance our understanding of conditions in which metabolism is altered (e.g., diabetes, cancer cachexia, failure to thrive and travel at zero-gravity). (C) 2003 Elsevier Inc. All rights reserved.