Structural chromosome 1 aberrations in transitional cell carcinoma of the bladder: interphase cytogenetics combining a centromeric, telomeric, and library DNA probe.
Structural chromosome 1 aberrations in transitional cell carcinoma of the bladder: interphase cytogenetics combining a centromeric, telomeric, and library DNA probe.
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发表时间：
1992-09
期刊：
影响因子：
11.2
通讯作者：
P. Poddighe;F. Ramaekers;A. Smeets;G. Vooijs;A. Hopman
中科院分区：
文献类型：
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作者：
P. Poddighe;F. Ramaekers;A. Smeets;G. Vooijs;A. Hopman
应用荧光原位杂交技术（FISH）研究膀胱移行细胞癌（tcc）间期细胞核中1号染色体的数量畸变和结构畸变。先前在低级别非侵袭性tcc中检测到的特征性数值畸变之一包括1号染色体三体（A. H. N. Hopman et al., Cancer, 51: 644-651, 1991）。我们使用着丝粒（1q12）和端粒相关（1p36） DNA探针以及从人类1号染色体分类的文库DNA探针，在单靶点和双靶点FISH程序中对22例进行了更详细的检查。流式细胞术检测的所有DNA二倍体tcc（13例）显示3个位点为1q12（6例），有2个位点为1p36。由于文库DNA探针在这些病例的细胞核中显示了三个独立的结构域，1q12的额外拷贝可以解释为额外的染色体1p-，包含1q12的目标。在流式细胞术测定的DNA四倍体/非整倍体肿瘤中，结果更为复杂。在9个病例中，我们观察到与1p36相比，1q12的代表性过高，这也表明存在额外的1p-染色体拷贝。本研究的结果证明了FISH方法在评估实体瘤间期细胞核结构染色体畸变方面的实用性。
Fluorescence in situ hybridization (FISH) was used to study numerical and structural chromosome 1 aberrations in interphase nuclei of transitional cell carcinomas (TCCs) of the urinary bladder. One of the characteristic numerical aberrations, as detected previously in low-grade noninvasive TCCs, included trisomy for chromosome 1 (A. H. N. Hopman et al., Cancer Res., 51: 644-651, 1991). We examined in more detail 22 cases with a centromeric (1q12) and a telomeric associated (1p36) DNA probe and with a library DNA probe from sorted human chromosome 1 in single- and double-target FISH procedures. All flow cytometrically determined DNA diploid TCCs (13 cases), which showed three spots for 1q12 (6 cases), had two spots for 1p36. Since the library DNA probe showed three separate domains in the nuclei of these cases, the additional copy for 1q12 could be explained as an extra chromosome 1p-, containing the 1q12 target. In the flow cytometrically determined DNA tetraploid/aneuploid tumors, the results were more complex. In 6 of 9 cases, we observed an overrepresentation of 1q12 as compared to 1p36, also suggesting the presence of extra copies of 1p- chromosomes. The results of the present study demonstrate the utility of the FISH method to assess structural chromosome aberrations in interphase nuclei of solid tumors.