Volumetric stress-strain analysis of optohydrodynamically suspended biological cells.
Volumetric stress-strain analysis of optohydrodynamically suspended biological cells.
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光流体动力学悬浮生物细胞的体积应力应变分析。
DOI：
10.1115/1.4002939
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Saha,AsitK
中科院分区：
文献类型：
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作者：
Kohles,SeanS;Liang,Yu;Saha,AsitK
正在进行的研究正在探索分离和悬浮生物细胞的生物力学特性，以追求对单细胞机械生物学的理解。一种应用最小激光功率的光钳将生物细胞定位在微流控交叉连接的几何中心，创建了一种新型的光流体陷阱。由此产生的流体流动环境促进了具有特定位置的法向和剪应力的单细胞的独特的多轴加载，从而导致了物理状态，尽管是拉伸状态。最近的一项二维分析探索了由这些流体诱导的应力引起的细胞骨骼应变响应[Wilson and Kohles，2010年，“二维纳米机械应力建模-微流体操作期间健康和患病单细胞中的应变”，J Nanotechnol Eng Med，1(2)，第021005页]。结果描述了具有可控纳米和微微牛顿分辨率的微流体环境。在本研究中，计算流体力学与多物理模型相结合，进一步表征了与实验细胞刺激相伴随的三维流体应力环境和固体细胞应变响应。对具有代表性的活细胞生物力学数据进行了体积应力应变分析。提出的法向和剪切应力表面图将指导未来的微流体实验，并为表征影响应力-应变响应的细胞骨架结构提供一个框架。
Ongoing investigations are exploring the biomechanical properties of isolated and suspended biological cells in pursuit of understanding single-cell mechanobiology. An optical tweezer with minimal applied laser power has positioned biologic cells at the geometric center of a microfluidic cross-junction, creating a novel optohydrodynamic trap. The resulting fluid flow environment facilitates unique multiaxial loading of single cells with site-specific normal and shear stresses resulting in a physical albeit extensional state. A recent two-dimensional analysis has explored the cytoskeletal strain response due to these fluid-induced stresses [Wilson and Kohles, 2010, “Two-Dimensional Modeling of Nanomechanical Stresses-Strains in Healthy and Diseased Single-Cells During Microfluidic Manipulation,” J Nanotechnol Eng Med, 1(2), p. 021005]. Results described a microfluidic environment having controlled nanometer and piconewton resolution. In this present study, computational fluid dynamics combined with multiphysics modeling has further characterized the applied fluid stress environment and the solid cellular strain response in three dimensions to accompany experimental cell stimulation. A volumetric stress-strain analysis was applied to representative living cell biomechanical data. The presented normal and shear stress surface maps will guide future microfluidic experiments as well as provide a framework for characterizing cytoskeletal structure influencing the stress to strain response.