Expression of vascular endothelial growth factor in canine mammary tumors
Expression of vascular endothelial growth factor in canine mammary tumors
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DOI：
10.1354/vp.39-4-488
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发表时间：
2002-07-01
影响因子：
2.4
通讯作者：
De Vico, G
中科院分区：
文献类型：
--
作者：
Restucci, B;Papparella, S;De Vico, G
血管内皮生长因子（VEGF）是一种二聚体蛋白质，通过诱导内皮细胞增殖和迁移在体内外刺激血管生成。本文应用免疫组化方法对10例犬乳腺良性肿瘤和40例犬乳腺恶性肿瘤的VEGF免疫标记细胞进行了计数，并对VEGF阳性的形态学特征（免疫标记强度、VEGF颗粒的Si/C值和分布）进行了评价。在良性肿瘤中检测到少量VEGF弱阳性细胞，具有少数和小颗粒极化到腔极。相反，在恶性肿瘤中，大量VEGF阳性细胞具有强免疫标记，通常在细胞质中弥漫发现大颗粒。这种免疫标记水平在分化程度较低、恶性程度较高的表型（3级）中更为明显。能够合成血管内皮生长因子的巨噬细胞呈强阳性。间质细胞和肌上皮细胞呈阴性。VEGF数据与肿瘤内微血管密度（新形成的微血管数量）在统计学上相关，两项指标在分化较低的恶性肿瘤中均较高，表明血管生成和恶性程度一起增加。VEGF在犬乳腺肿瘤中似乎是一种强有力的血管生成因子。
Vascular endothelial growth factor (VEGF) is a dimeric Protein that simulates angiogenesis in vitro and in vivo by inducing endothelial cell proliferation and migration. In this immunohistochemical study VEGF-immunolabeled cells were counted in a series of 10 benign and 40 malignant canine mammary, tumors, The morphologic pattern of VEGF positivity (intensity of immunolabeling and VEGF granule Si/C and distribution) was also evaluated. A low number of cells weakly positive for VEGF with few and small granules polarized to the luminal pole was detected in benign neoplasms. In contrast, in malignancies high number of VEGF-positive cells had strong immunolabeling, often with large granules found diffusely in the cytoplasm. This level of immunolabeling was more pronounced in the less differentiated, more malignant phenotypes (grade 3). Macrophages, which can synthesize VEGF, were strongly positive. Stromal and myoepithelial cells were negative. VEGF data were correlated statistically with intratumoral microvessel density (number of newly formed microvessels) and both measures were greater in less differentiated malignant neoplasms, demonstrating that angiogenesis and malignancy increase together. VEGF appears to be a powerful angiogenic factor in canine mammary tumors.