An outbreak of gastroenteritis by emerging norovirus GII.2[P16] in a kindergarten in Kota Kinabalu, Malaysian Borneo
An outbreak of gastroenteritis by emerging norovirus GII.2[P16] in a kindergarten in Kota Kinabalu, Malaysian Borneo
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DOI：
10.1038/s41598-020-64148-4
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发表时间：
2020-04-28
影响因子：
4.6
通讯作者：
Iha, Hidekatsu
中科院分区：
文献类型：
--
作者：
Ahmed, Kamruddin;Dony, Jiloris Julian Frederick;Iha, Hidekatsu
在发展中国家，幼儿园爆发的腹泻疫情被低估了，而且往往没有人注意到。为了更好地了解病因，这项研究是在马来西亚沙巴州一所幼儿园爆发腹泻期间进行的。疫情调查按标准程序进行。在这次暴发中，共有34名儿童(36.5%)和4名教师(30.8%)患上了胃肠炎。对7名儿童和13名教师的粪便样本进行了轮状病毒和诺沃克病毒检测。在调查期间，采集了粪便样本，并将其冷链送往实验室。对标本进行轮状病毒酶联免疫吸附试验和逆转录-聚合酶链式反应检测诺如病毒。所有样本轮状病毒均为阴性，但诺沃克病毒均呈阳性。为了确定诺如病毒的基因组和基因型，对扩增产物进行了核苷酸测序。此次暴发的所有诺沃克病毒均为GII.2[16]型。为确定菌株间的亲缘关系，采用邻接法进行系统发育分析。这些毒株与中国和日本的GII.2[P16]诺如病毒有较高的亲缘关系。这项研究提供的证据表明，一所幼儿园的腹泻暴发是由GII.2[P16]诺沃克病毒引起的，诺沃克病毒是东亚和欧洲的一种新毒株。
Outbreaks of diarrhea in kindergartens are underreported and frequently go unnoticed in developing countries. To better understand the etiology this study was performed during an outbreak of diarrhea in a kindergarten in Sabah, Malaysia. Outbreak investigation was performed according to the standard procedures. In this outbreak a total of 34 (36.5%) children and 4 (30.8%) teachers suffered from gastroenteritis. Stool samples from seven children and 13 teachers were tested for rotavirus and norovirus. During the investigation stool samples were collected and sent in cold chain to the laboratory. The samples were subjected to rotavirus enzyme linked immunosorbent assay, and reverse transcription PCR for norovirus. All samples were negative for rotavirus but positive for norovirus. To determine the genogroup and genotype of norovirus, nucleotide sequencing of the amplicons was performed. All norovirus from the outbreak was of genotype GII.2[16]. To determine the relatedness of the strains phylogenetic analysis was done using neighbor-joining method. Phylogenetically these strains were highly related to GII.2[P16] noroviruses from China and Japan. This study provided evidence that a diarrheal outbreak in a kindergarten was caused by GII.2[P16] norovirus which is an emerging strain in East Asia and Europe.