Expression of Sox transcription factors in the developing mouse pancreas
Expression of Sox transcription factors in the developing mouse pancreas
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DOI：
10.1002/dvdy.10311
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发表时间：
2003-07-01
影响因子：
2.5
通讯作者：
Sander, M
中科院分区：
文献类型：
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作者：
Lioubinski, O;Müller, M;Sander, M
先前的工作已经确定了同源结构域和基本螺旋-环-螺旋转录因子家族的成员是哺乳动物胰腺发育的关键决定因素。在这里，我们描述了小鼠胰腺中Sox基因家族的HMG-box转录因子的鉴定。我们在胚胎胰腺中检测到了Sox11、Sox4、Sox13、Sox5、Sox9、Sox8、Sox10、Sox7、Sox17、Sox18、Sox15和Sox30的转录本，并在成人胰岛中发现了Sox4、Sox9和Sox13。从早期胰腺生长到出生，通过原位杂交更详细地研究了其中7个Sox因子的表达。Sox11在胰腺芽周围的间质中表达，而Sox4和Sox9则局限于胰腺上皮，后来转移到胰岛。Sox13和L-Sox5在胚胎12.5 ~ 14.5天大部分胰腺上皮细胞中表达。在胰岛周围的细胞薄层中检测到Sox8和Sox10。Sox基因在胚胎胰腺中的表达模式表明，它们可能在胰腺发育中具有重要且可能冗余的功能。(C) 2003 Wiley-Liss, Inc。
Previous work has identified members of the homeodomain and basic helix-loop-helix families of transcription factors as critical determinants of mammalian pancreatic development. Here, we describe the identification of HMG-box transcription factors of the Sox gene family in the mouse pancreas. We detected transcripts for Sox11, Sox4, Sox13, Sox5, Sox9, Sox8, Sox10, Sox7, Sox17, Sox18, Sox15, and Sox30 in embryonic pancreas and found Sox4, Sox9, and Sox13 in adult pancreatic islets. Expression of seven of these Sox factors was studied in more detail by in situ hybridization from the stage of early pancreatic outgrowth to birth. Expression of Sox11 was found in the mesenchyme surrounding the pancreatic buds, whereas Sox4 and Sox9 were confined to the pancreatic epithelium and later to islets. Sox13 and L-Sox5 showed expression in most of the pancreatic epithelial cells between embryonic days 12.5 and 14.5. Sox8 and Sox10 were detected in a thin layer of cells surrounding the islets. The expression patterns of Sox genes in the embryonic pancreas suggest that they could have important and possibly redundant functions in pancreas development. (C) 2003 Wiley-Liss, Inc.