Paraoxonase 55 and 192 polymorphism and its relationship to serum paraoxonase activity and serum lipids in Turkish patients with non-insulin dependent diabetes mellitus
Paraoxonase 55 and 192 polymorphism and its relationship to serum paraoxonase activity and serum lipids in Turkish patients with non-insulin dependent diabetes mellitus
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DOI：
10.1002/cbf.1070
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发表时间：
2004-05-01
影响因子：
3.6
通讯作者：
Isbir, T
中科院分区：
文献类型：
--
作者：
Agachan, B;Yilmaz, H;Isbir, T
我们调查了213名土耳其非胰岛素依赖型糖尿病（NIDDM）患者和116名非糖尿病对照者的PON 55和PON 192多态性对血清PON 1活性和血脂谱的影响。采用聚合酶链反应-限制性片段长度多态性方法检测PON 55/192基因多态性分布。用酶法测量血清脂质水平。通过分光光度法测定加入对氧磷后对硝基苯酚的产生来测量PON活性。我们发现PON 55和192基因型在糖尿病患者和对照组中的分布相似，糖尿病患者的对氧磷酶活性普遍低于对照组。我们发现PON 55和192基因型对血清PON活性有主要影响。在对照组和NIDDM人群中，PON 192 BB基因型的PON活性显著高于AA和AB基因型（p < 0.001）。在对照组和NIDDM人群中，PON 55 MM纯合子的PON活性显著低于LL和LM基因型（p < 0.05）。PON 1 55和192多态性并不影响两个人群的血脂谱。总之，我们的研究结果表明，对氧磷酶的活动受到影响的PON我在土耳其NIDDM患者和对照组的遗传变异。版权所有（C）2004约翰威利父子有限公司。
We investigated the effect of PON 55 and PON 192 polymorphisms on serum PON1 activity and lipid profiles in 213 non-insulin dependent diabetes mellitus (NIDDM) individuals and 116 non-diabetic controls among Turkish subjects. The distribution of PON 55/192 gene polymorphism was determined by polymerase chain reaction-based restriction fragment length polymorphism. Serum lipid levels were measured enzymically. PON activity was measured by spectrophotometric assay of p-nitrophenol production following addition of paraoxon. We found that PON 55 and 192 genotype distribution was similar in patients and controls and paraoxonase activity was generally lower in diabetics than in control subjects. We showed that PON 55 and 192 genotypes have a major effect on serum PON activity. PON 192 BB homozygotes had significantly higher PON activity than AA and AB genotypes among the control and NIDDM populations (p < 0.001). PON 55 MM homozygotes had significantly lower PON activity than did LL and LM genotypes in control and NIDDM populations (p < 0.05). The PON1 55 and 192 polymorphisms did not consistently influence the serum lipid profiles in either population. In conclusion, our results suggest that the paraoxonase activities are affected by PON I genetic variability in Turkish NIDDM patients and controls. Copyright (C) 2004 John Wiley Sons, Ltd.