Isolation and characterization of the human pulmonary surfactant apoprotein gene
Isolation and characterization of the human pulmonary surfactant apoprotein gene
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DOI：
10.1038/317361a0
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发表时间：
1985
期刊：
影响因子：
64.8
通讯作者：
R. T. White;D. Damm;Judy Miller;K. Spratt;J. Schilling;S. Hawgood;B. Benson;B. Cordell
中科院分区：
文献类型：
--
作者：
R. T. White;D. Damm;Judy Miller;K. Spratt;J. Schilling;S. Hawgood;B. Benson;B. Cordell
肺表面活性物质是一种磷脂-蛋白质复合物，用于降低哺乳动物肺泡中气-液界面的表面张力，对正常呼吸至关重要1。出生时表面活性剂水平不足，这是早产儿常见的情况，会导致呼吸衰竭。在所有检查的物种中，表面活性剂主要由二棕榈酰磷脂酰胆碱和相对分子质量（Mr）为32，000（32 K）和10 K的两种主要蛋白质组成（参考文献2-5）。用合成脂质在体外重构纯化的32 K肺表面活性物质脱辅基蛋白（PSAP），形成脂蛋白复合物，通过铺展产生薄的界面膜6，7降低表面张力。在这里，我们描述了克隆的人PSAP基因和互补DNA，并讨论了不寻常的编码蛋白质的特点。
Pulmonary surfactant is a phospholipid–protein complex which serves to lower the surface tension at the air–liquid interface in the alveoli of the mammalian lung and is essential for normal respiration1. Inadequate levels of surfactant at birth, a frequent situation in premature infants, results in respiratory failure. In all species examined, surfactant is composed primarily of dipalmitoylphosphatidylcholine and two major protein species of relative molecular mass (Mr) 32,000 (32K) and 10K (refs 2–5). Reconstitutionin vitroof purified 32K pulmonary surfactant apoprotein (PSAP) with synthetic lipids forms a lipoprotein complex that lowers surface tension by spreading to create a thin interfacial film6,7. Here we describe the cloning of the human PSAP gene and complementary DNA, and discuss features of the unusual encoded protein.