High-Yield Production of Propionate from 1,2-Propanediol by Engineered Pseudomonas putida KT2440, a Robust Strain with Highly Oxidative Capacity.
High-Yield Production of Propionate from 1,2-Propanediol by Engineered Pseudomonas putida KT2440, a Robust Strain with Highly Oxidative Capacity.
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DOI：
10.1021/acs.jafc.2c06405
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发表时间：
2022-12
影响因子：
6.1
通讯作者：
Ya'nan Shi;Rongshan Li;Jie Zheng;Yubin Xue;Yong Tao;Bo Yu
中科院分区：
文献类型：
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作者：
Ya'nan Shi;Rongshan Li;Jie Zheng;Yubin Xue;Yong Tao;Bo Yu
生物基丙酸酯因其绿色特性和特定的食品添加剂市场而受到越来越多的关注。到目前为止，严格的厌氧菌发酵过程既耗时又昂贵，使丙酸酯的生产并不理想。本研究利用恶臭假单胞菌KT2440菌株较强的氧化能力，设计了一条先将1，2-丙二醇脱水生成丙醛再生成丙酸的新工艺。该路线具有较高的原子经济性(0.97g/g)，比以前的L-苏氨酸路线(0.62g/g)更具优势。首次对恶臭假单胞菌KT2440超强氧化能力的分子机制进行了破译。恶臭假单胞菌KT2440通过筛选合适的甘油脱水酶并对其表达进行优化，消除了丙醇的生成和中间产物丙醛的积累，从而实现了丙酸的生产。该工程菌以1，2-丙二醇为原料合成丙酸酯，摩尔转化率为>；99%。在补料间歇生物转化中，丙酸的效价为46.5g/L，产率为1.55g/L/h，质量产率为0.96g/g。因此，本研究为利用原子经济性高的可再生材料生产高纯度生物基丙酸酯提供了另一种思路。
Bio-based propionate attracts increasing attention owing to its green nature and specific food additive market. To date, the time-consuming and costly fermentation process by strict anaerobes makes propionate production not ideal. In this study, we designed a new route for propionate production, in which 1,2-propanediol was first dehydrated to propionaldehyde and then to propionate by taking advantage of the robust oxidization capacity of the Pseudomonas putida KT2440 strain. The high atom economy (0.97 g/g) in this proposed pathway is more advantageous than the previous l-threonine-derived route (0.62 g/g). The molecular mechanism of the extraordinary oxidation capacity of P. putida KT2440 was first deciphered. The propionate production was realized in P. putida KT2440 by screening suitable glycerol dehydratases and optimizing the expression to eliminate the formation of 1-propanol and the accumulation of the intermediate propionaldehyde. The engineered strain produced propionate with a molar conversion rate of >99% from 1,2-propanediol. A high titer of 46.5 g/L pure propionic acid with a productivity of 1.55 g/L/h and a mass yield of 0.96 g/g was achieved in fed-batch biotransformation. Thus, this study provides another idea for the production of high-purity bio-based propionate from renewable materials with high atom economy.