Quantitative Dextran Trafficking to the Coxiella burnetii Parasitophorous Vacuole.
Quantitative Dextran Trafficking to the Coxiella burnetii Parasitophorous Vacuole.
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DOI：
10.1002/cpmc.34
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发表时间：
2017-08-11
影响因子：
--
通讯作者：
Gilk SD
中科院分区：
文献类型：
--
作者：
Winfree S;Gilk SD
The gram-negative bacterium Coxiella burnetii causes human Q fever, a disease characterized by a debilitating flu-like illness in acute cases and endocarditis in chronic patients. An obligate intracellular pathogen, Coxiella burnetii survives within a large, lysosome-like vacuole inside the host cell. A unique feature of the Coxiella parasitophorous vacuole (PV) is high levels of fusion with the host endocytic pathway, with PV-endosome fusion critical for Coxiella survival within the host cell. This unit describes quantitating PV-endosome fusion by measuring delivery of the fluid phase endosome marker dextran to the PV using live cell imaging. To study the effect of host cell proteins involved in PV-endosome fusion, details are provided for using siRNA knockdown host cells. This method is a powerful tool to understanding mechanisms underlying Coxiella’s ability to manipulate host cell trafficking pathways.
