Establishment of a primary hepatocyte culture from the small Indian mongoose (Herpestes auropunctatus) and distribution of mercury in liver tissue
Establishment of a primary hepatocyte culture from the small Indian mongoose (Herpestes auropunctatus) and distribution of mercury in liver tissue
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DOI：
10.1007/s10646-014-1307-6
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发表时间：
2014-11-01
期刊：
影响因子：
2.7
通讯作者：
Furukawa, Tatsuhiko
中科院分区：
文献类型：
--
作者：
Horai, Sawako;Yanagi, Kumiko;Furukawa, Tatsuhiko
本研究建立了原代肝细胞培养的小印度猫鼬（疱疹auropunctatus）。为了确定适合该物种原代肝细胞生长的培养基，我们比较了在三种常用于哺乳动物细胞培养的培养基中培养的细胞的条件：Dulbecco改良Eagle培养基、RPMI-1640和William's E.其中，William's E培养基最适合于培养该物种的肝细胞。采用过碘酸-希夫染色和超微结构观察，证实所采集的猫鼬肝细胞为肝细胞。为了评价汞在肝组织中的分布，我们进行了自显影染色。汞化合物主要分布在肝小叶中央。平滑内质网在异生物质代谢、脂质/胆固醇代谢以及亲脂性物质的消化和解毒中起作用。提示Hg与滑面内质网共定位。本研究结果可为高汞含量野生动物体内解毒系统的鉴定和对汞毒性敏感性的评价提供参考。
The present study established a primary hepatocyte culture for the small Indian mongoose (Herpestes auropunctatus). To determine the suitable medium for growing the primary hepatic cells of this species, we compared the condition of cells cultured in three media that are frequently used for mammalian cell culture: Dulbecco's Modified Eagle's Medium, RPMI-1640, and William's E. Of these, William's E medium was best suited for culturing the hepatic cells of this species. Using periodic acid-Schiff staining and ultrastructural observations, we demonstrated the cells collected from mongoose livers were hepatocytes. To evaluate the distribution of mercury (Hg) in the liver tissue, we carried out autometallography staining. Most of the Hg compounds were found in the central region of hepatic lobules. Smooth endoplasmic reticulum, which plays a role inxenobiotic metabolism, lipid/cholesterol metabolism, and the digestion and detoxification of lipophilic substances is grown in this area. This suggested that Hg colocalized with smooth endoplasmic reticulum. The results of the present study could be useful to identify the detoxification systems of wildlife with high Hg content in the body, and to evaluate the susceptibility of wildlife to Hg toxicity.