COMPARATIVE GENOMIC ANALYSIS OF TUMORS - DETECTION OF DNA LOSSES AND AMPLIFICATION
COMPARATIVE GENOMIC ANALYSIS OF TUMORS - DETECTION OF DNA LOSSES AND AMPLIFICATION
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DOI：
10.1073/pnas.92.1.151
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发表时间：
1995-01-03
影响因子：
11.1
通讯作者：
WIGLER, MH
中科院分区：
文献类型：
--
作者：
LISITSYN, NA;LISITSINA, NM;WIGLER, MH
我们展示了使用代表性差异分析克隆探针，检测肿瘤中的DNA丢失和扩增。使用从人类肿瘤细胞系中分离的DNA来驱动与匹配的正常DNA的杂交，我们能够鉴定出在培养的癌细胞中同源缺失的六个基因组区域。当以相反的方式应用该方法时，使用正常DNA驱动针对肿瘤细胞DNA的杂交，我们容易分离检测扩增的探针。代表性差异分析也来自肿瘤活检的DNA进行，从而我们发现了一个探针检测非常频繁的纯合性损失，在结肠癌细胞系和位于染色体3p。
We demonstrate the use of representational difference analysis for cloning probes that detect DNA loss and amplification in tumors. Using DNA isolated from human tumor cell lines to drive hybridization against matched normal DNA, we were able to identify six genomic regions that are homozygously deleted in cultured cancer cells. When this method was applied in the reverse way, using normal DNA to drive hybridization against tumor cell DNA, we readily isolated probes detecting amplification. Representational difference analysis was also performed on DNAs derived from tumor biopsies, and we thereby discovered a probe detecting very frequent homozygous loss in colon cancer cell lines and located on chromosome 3p.