Microbiome assembly across multiple body sites in low-birthweight infants.
Microbiome assembly across multiple body sites in low-birthweight infants.
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DOI：
10.1128/mbio.00782-13
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发表时间：
2013-10-29
期刊：
影响因子：
6.4
通讯作者：
Relman DA
中科院分区：
文献类型：
--
作者：
Costello EK;Carlisle EM;Bik EM;Morowitz MJ;Relman DA
本研究的目的是评估与低出生体重儿（LBW）相关的细菌群落的组成和丰富度与宿主身体部位，个体和年龄的关系。从出生后第8、10、12、15、18和21天从6名LBW（5名早产）婴儿收集的唾液样本、皮肤拭子和粪便样本中的细菌16 S rRNA基因进行扩增、焦磷酸测序，并在包括正常出生体重（NBW）婴儿和健康成人的类似数据的比较框架内进行分析。我们发现，身体部位是LBW婴儿细菌群落组成的主要决定因素。然而，部位特异性取决于出生后的年龄：唾液和粪便成分随着时间的推移而不同，但直到婴儿15天大时才有显着差异。这种差异主要是由远端肠道的进行性时间转换驱动的，其速率与年龄匹配的NBW婴儿相似。新生儿皮肤的微生物群组成最像成年人，而唾液和粪便仍然最不像成年人。婴儿之间的成分变化是显着的，并依赖于身体部位和年龄。在研究期间，只有最小、最早产的婴儿接受了抗生素治疗;这预示着铜绿假单胞菌和新型支原体在这位阴道分娩、插管患者的口腔中共同扩增。我们的结论是，LBW新生儿多个身体部位的同步分子监测揭示了口腔和远端肠道微生物群的延迟组成分化，在一个婴儿的情况下，丰富的，未培养的口腔支原体，最近在人类阴道样本中检测到。早产并发症是新生儿死亡的最常见原因。在分娩时开始的本土微生物群的定植可能使一些高风险新生儿易患侵袭性感染或坏死性小肠结肠炎（NEC），并保护其他新生儿，但新生儿微生物组动力学知之甚少。在这里，我们提出了第一个独立于培养的时间序列，跟踪住院低出生体重（LBW）新生儿同步队列中多个身体部位的微生物群组装。我们利用存档的样本和实验室可用的序列数据，并将我们的LBW婴儿结果与正常出生体重（NBW）婴儿和健康成人的结果进行比较。我们的研究结果表明，微生物在宿主内和宿主之间传播的潜在机会窗口，并支持最近发现的高风险新生儿微生物群组成的大量基线时空变化。
The purpose of this study was to evaluate the composition and richness of bacterial communities associated with low-birthweight (LBW) infants in relation to host body site, individual, and age. Bacterial 16S rRNA genes from saliva samples, skin swabs, and stool samples collected on postnatal days 8, 10, 12, 15, 18, and 21 from six LBW (five premature) infants were amplified, pyrosequenced, and analyzed within a comparative framework that included analogous data from normal-birthweight (NBW) infants and healthy adults. We found that body site was the primary determinant of bacterial community composition in the LBW infants. However, site specificity depended on postnatal age: saliva and stool compositions diverged over time but were not significantly different until the babies were 15 days old. This divergence was primarily driven by progressive temporal turnover in the distal gut, which proceeded at a rate similar to that of age-matched NBW infants. Neonatal skin was the most adult-like in microbiota composition, while saliva and stool remained the least so. Compositional variation among infants was marked and depended on body site and age. Only the smallest, most premature infant received antibiotics during the study period; this heralded a coexpansion of Pseudomonas aeruginosa and a novel Mycoplasma sp. in the oral cavity of this vaginally delivered, intubated patient. We conclude that concurrent molecular surveillance of multiple body sites in LBW neonates reveals a delayed compositional differentiation of the oral cavity and distal gut microbiota and, in the case of one infant, an abundant, uncultivated oral Mycoplasma sp., recently detected in human vaginal samples. Complications of premature birth are the most common cause of neonatal mortality. Colonization by the indigenous microbiota, which begins at delivery, may predispose some high-risk newborns to invasive infection or necrotizing enterocolitis (NEC), and protect others, yet neonatal microbiome dynamics are poorly understood. Here, we present the first cultivation-independent time series tracking microbiota assembly across multiple body sites in a synchronous cohort of hospitalized low-birthweight (LBW) neonates. We take advantage of archived samples and publically available sequence data and compare our LBW infant findings to those from normal-birthweight (NBW) infants and healthy adults. Our results suggest potential windows of opportunity for the dispersal of microbes within and between hosts and support recent findings of substantial baseline spatiotemporal variation in microbiota composition among high-risk newborns.