Proton-detected solid-state NMR detects the inter-nucleotide correlations and architecture of dimeric RNA in microcrystals
Proton-detected solid-state NMR detects the inter-nucleotide correlations and architecture of dimeric RNA in microcrystals
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质子检测固态 NMR 可检测微晶中二聚体 RNA 的核苷酸间相关性和结构
DOI：
10.1039/c7cc07483b
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发表时间：
2017
影响因子：
4.9
通讯作者：
Wang Shenlin
中科院分区：
文献类型：
--
作者：
Yang Yufei;Xiang ShengQi;Liu Xiaodan;Pei Xiaojing;Wu Pengzhi;Gong Qingguo;Li Na;Baldus Marc;Wang Shenlin
我们报告了一种基于 15N-15N 质子辅助重偶联的新型质子检测 MAS 固态 NMR 策略，用于检测微晶二聚体 RNA 的 Watson-Crick 碱基对内的核苷酸间 NH⋯N 氢键，并确认接吻环结构。这将有助于利用固态 NMR 确定 RNA 结构的进展。
We report a novel proton-detected MAS solid-state NMR strategy based on 15N–15N proton assisted recoupling to detect the inter-nucleotide NH⋯N hydrogen bonds within the Watson–Crick base pairs of micro-crystallized dimeric RNA and to confirm the kissing-loop structure. This would contribute to advances in the structural determination of RNA using solid-state NMR.