Well-ordered chitin whiskers layer with high stability on the surface of poly (D, L-lactide) film for enhancing mechanical and osteogenic properties
Well-ordered chitin whiskers layer with high stability on the surface of poly (D, L-lactide) film for enhancing mechanical and osteogenic properties
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聚（D，L-丙交酯）薄膜表面具有高度稳定性的有序甲壳晶须层，可增强机械和成骨性能
DOI：
10.1016/j.carbpol.2019.02.060
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发表时间：
2019
影响因子：
11.2
通讯作者：
Luo Binghong
中科院分区：
文献类型：
--
作者：
Liu Wenjun;Zhu Ling;Ma Yunfa;Ai Lihao;Wen Wei;Zhou Changren;Luo Binghong
为了更好地利用和结合聚(d，L-丙交酯)(PDLLA)基质和天然多糖甲壳素晶须(CHWS)的优点，选择了具有手性向列液晶特性和显著模数的针状CHWS对PDLLA薄膜进行表面修饰。通过垂直涂覆的方法，通过聚多巴胺(PDA)夹层在PDLLA薄膜表面形成了稳定有序的CHWS涂层。通过PBS浸泡实验考察了薄膜质量和表面形貌的变化，证实了PDLLA薄膜表面CHWS涂层具有良好的稳定性。用场发射扫描电子显微镜(FESEM)、原子力显微镜(AFM)和X射线光电子能谱(XPS)对PDLLA-PDA-CHWS薄膜的表面结构和成分进行了表征，并用场发射扫描电子显微镜测定了PDLLA、PDA和CHWS薄膜的厚度。与传统的溶液共混法制备的PDLLA/CHWS膜相比，当CHWS的最佳含量为5 wt%时，由于CHWS涂层高度有序的固定，使膜的亲水性和力学性能显著提高。此外，体外细胞培养表明，有序的CHWS涂层对MC3T3-E1细胞的黏附、铺展、增殖和成骨分化有更明显的促进作用。这些结果表明，通过简单有效的方法设计出具有有序CHWS涂层的PDLLA-PDA-CHWS薄膜，在骨修复材料领域具有良好的应用前景。
In order to better utilize and combine the advantages of poly(d,l-lactide) (PDLLA) matrix and natural polysaccharide chitin whiskers (CHWs), needle-like CHWs with chiral nematic liquid crystal characteristics and remarkable moduli were chosen to surface modify PDLLA film. By a vertical coating method, a stable and well-ordered CHWs coating was formed on the surface of the PDLLA film through a polydopamine (PDA) interlayer. The high stability of the well-ordered CHWs coating on the surface of the PDLLA film was confirmed by PBS soaking experiment in terms of the changes of weight and surface morphology of the film. The surface microstructure and composition of the resulting PDLLA-PDA-CHWs film were characterized by field emission scanning electron microscopy (FESEM), atomic force microscope (AFM) and X-ray photoelectron spectroscopy (XPS), and the thickness of the PDLLA, PDA and CHWs layers was determined using FESEM, too. Comparing with traditional PDLLA/CHWs film, prepared by solution blending with optimal content of 5 wt% CHWs, the hydrophilicity and mechanical properties of the PDLLA-PDA-CHWs film were significantly improved owing to the immobilization of a highly ordered CHWs coating. Furthermore, in vitro cell culture showed that the well-ordered CHWs coating had a more significant effect on promoting the adhesion, spreading, proliferation and osteogenic differentiation of MC3T3-E1 cells. All these results showed that the resulting PDLLA-PDA-CHWs film with well-ordered CHWs coating designed through simple and effective approach has promising application in bone repair material field.