Accelerated aging and nucleolar fragmentation yeast sgs1 mutants
Accelerated aging and nucleolar fragmentation yeast sgs1 mutants
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DOI：
10.1126/science.277.5330.1313
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发表时间：
1997-08-29
期刊：
影响因子：
56.9
通讯作者：
Guarente, L
中科院分区：
文献类型：
--
作者：
Sinclair, DA;Mills, K;Guarente, L
酵母的SGS1基因编码与人WRN基因同源的DNA解旋酶。WRN的突变导致沃纳综合征，一种症状类似早衰的疾病。SGS1的突变被证明会导致酵母母细胞的寿命缩短和衰老诱导的不育表型以及Sir3沉默蛋白从端粒到核仁的再分布的基础上过早衰老。此外，在年老的sgs1细胞中，核仁扩大并碎裂，这种变化也发生在年老的野生型细胞中。这些发现表明细胞衰老的保守机制可能与核仁结构有关。
The SGS1 gene of yeast encodes a DNA helicase with homology to the human WRN gene. Mutations in WRN result in Werner's syndrome, a disease with symptoms resembling premature aging. Mutation of SGS1 is shown to cause premature aging in yeast mother cells on the basis of a shortened life-span and the aging-induced phenotypes of sterility and redistribution of the Sir3 silencing protein from telomeres to the nucleolus. Further, in old sgs1 cells the nucleolus is enlarged and fragmented-changes that also occur in old wild-type cells. These findings suggest a conserved mechanism of cellular aging that may be related to nucleolar structure.