Ovine amniotic fluid volume response to intra-amniotic balloon filling.
Ovine amniotic fluid volume response to intra-amniotic balloon filling.
复制标题
绵羊羊水量对羊膜内球囊充盈的反应。
DOI：
10.1016/j.placenta.2008.11.015
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发表时间：
2009
期刊：
影响因子：
3.8
通讯作者：
Anderson,DF
中科院分区：
文献类型：
--
作者：
Faber,JJ;Brace,RA;Davis,LE;Anderson,DF
虽然羊胎盘与人类胎盘有明显的不同，但羊模型已被广泛用于研究羊水容量的调节，因为大多数实验都是在长期插管的胎羊中进行的。然而，在羊水体积、胎儿排尿和胎儿吞咽方面，人胎和羊胎有相当多的相似之处。在这两个物种中，羊水在整个妊娠期都有持续的周转。在绵羊中，尿液和肺液进入羊水，而羊水通过吞咽和羊膜膜内吸收被排出[1,2]。因为羊水的体积保持恒定在0.5-1.0升，尽管每天的周转量约为1升，体积必须调节。羊水的调节可由机械传感器（如子宫或膜的拉伸）或化学传感器（如羊水中效应物质浓度的改变）介导。下面描述的是我们研究羊水体积对羊膜内气囊填充引起的宫内总体积变化的反应的实验结果。该方案得到了OHSU机构动物护理和使用委员会的批准。对6只妊娠120天的妊娠羊（5只单胎羊和1只双胞胎羊，其中只有一只使用仪器）进行了手术。这些制备的细节已经在前面描述过[3,4]。简单地说，在每个胎儿的股动脉和股静脉中放置导管。在膀胱颈部结扎尿管以防止尿液进入尿囊腔。将三根羊水引流导管和两根羊水压力测量导管附着在胎体上。最后，一个或两个
Although ovine placentation is markedly different from human, the ovine model has been extensively used to study the regulation of amniotic fluid volume because the majority of the experiments has been performed in the chronically catheterized fetal sheep. However, there are considerable similarities between the human fetus and the sheep fetus with regard to amniotic fluid volumes, fetal urination and fetal swallowing. In both species, there is a continual turnover of amniotic fluid throughout gestation. In sheep, urine and lung fluids enter the amniotic fluid while fluid is removed through swallowing and intramembranous absorption across the amniochorion [1, 2]. Because the amniotic fluid volume remains constant at about 0.5–1.0 l in spite of a turnover of about 1 l per day, the volume must be regulated. Regulation of the amniotic fluid may be mediated by a mechanical sensor (such as stretch of the uterus or membranes) or a chemical sensor (such as an alteration in the concentration of an effector substance in amniotic fluid). Described below are results from our experiments examining the response of amniotic fluid volume to changes in total intrauterine volume induced by filling of an intra-amniotic balloon.The protocol received approval of the OHSU Institutional Animal Care and Use Committee. Surgeries were performed on 6 pregnant sheep (5 singletons and 1 twin, only one of which was instrumented) at 120 days of gestation. The details of these preparations have been previously described [3, 4]. Briefly, catheters were placed in the femoral artery and vein of each fetus. The urachus was ligated at the neck of the bladder to prevent urine from entering the allantoic space. Three catheters for draining amniotic fluid and two catheters for measuring the amniotic fluid pressure were attached to the fetal body. Finally, one or two