The Majchrowicz binge alcohol protocol: an intubation technique to study alcohol dependence in rats.
The Majchrowicz binge alcohol protocol: an intubation technique to study alcohol dependence in rats.
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DOI：
10.1002/0471142301.ns0928s44
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发表时间：
2008-07-01
影响因子：
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通讯作者：
Faingold, Carl L
中科院分区：
文献类型：
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作者：
Faingold, Carl L
酗酒是一个重要的公共卫生问题，需要进行实验研究，以了解这个问题的病理生理机制，并制定改进的治疗方法。本单元提出了一个有效的和广泛使用的方法来模拟酗酒的大鼠。它包括每天三次对大鼠胃内给予乙醇，持续4天。乙醇最初以5 g/kg给药，然后根据每次给药前大鼠表现出的中毒程度确定每次后续剂量。动物的行为根据描述良好的量表进行分级。在第四天之后，可以在可预测的时间过程中观察到乙醇戒断综合征的各个方面，并且可以采用额外的方案来研究戒断的特定方面的机制和预防它们的治疗。在乙醇戒断过程中观察到的一种特殊行为是声音诱导的（听源性）惊厥发作。
Binge drinking of alcohol is an important public health issue, and experimental studies are needed to understand the pathophysiological mechanisms of this problem and develop improved approaches to treatment. This unit presents a validated and widely used method to model binge alcohol drinking in rats. It consists of three daily intragastric administrations of ethanol to rats for 4 days. Ethanol is initially administered at 5 g/kg, and then each subsequent dose is determined based on the degree of intoxication the rat exhibits prior to each dose. The behavior of the animal is graded based on a well-described scale. After the fourth day, various aspects of the ethanol withdrawal syndrome can be observed over a predictable time course and additional protocols can be employed to study mechanisms of specific aspects of withdrawal and treatments to prevent them. One specific behavior observed during ethanol withdrawal and described here is sound-induced (audiogenic) convulsive seizure.