Nucleoid remodeling by an altered HU protein: Reorganization of the transcription program
Nucleoid remodeling by an altered HU protein: Reorganization of the transcription program
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DOI：
10.1073/pnas.0508032102
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发表时间：
2005-11-08
影响因子：
11.1
通讯作者：
Adhya, S
中科院分区：
文献类型：
--
作者：
Kar, S;Edgar, R;Adhya, S
细菌类核组织被认为对全局转录程序的影响最小。通过改变细菌组蛋白样蛋白HU α，我们发现类核结构的重组可以动态调节细胞转录模式。突变蛋白将松散排列的类核转变为紧密凝聚的结构。类核压实，加上全球DNA超卷曲的增加，在野生型K-12大肠杆菌的形态、生理和代谢方面产生了根本性的变化。在突变体中，许多与养分利用有关的构成管家基因被抑制，而与毒力相关的许多静止基因被激活。我们认为，与真核生物一样，类核结构决定了细菌的全局转录谱，从而决定了细菌的行为模式。
Bacterial nucleoid organization is believed to have minimal influence on the global transcription program. Using an altered bacterial histone-like protein, HU alpha, we show that reorganization of the nucleoid configuration can dynamically modulate the cellular transcription pattern. The mutant protein transformed the loosely packed nucleoid into a densely condensed structure. The nucleoid compaction, coupled with increased global DNA supercoiling, generated radical changes in the morphology, physiology, and metabolism of wild-type K-12 Escherichia coli. Many constitutive housekeeping genes involved in nutrient utilization were repressed, whereas many quiescent genes associated with virulence were activated in the mutant. We propose that, as in eukaryotes, the nucleoid architecture dictates the global transcription profile and, consequently, the behavior pattern in bacteria.