Measuring activation patterns of the heart at a microscopic size scale with thin-film sensors.
Measuring activation patterns of the heart at a microscopic size scale with thin-film sensors.
复制标题
使用薄膜传感器在微观尺寸范围内测量心脏的激活模式。
DOI：
10.1152/ajpheart.1994.266.5.h2136
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Platzer,D
中科院分区：
文献类型：
--
作者：
Hofer,E;Urban,G;Spach,MS;Schafferhofer,I;Mohr,G;Platzer,D
为了研究心室准备中兴奋的传播，我们设计了一个快速，高分辨率的记录和标测系统。从离体豚鼠心脏解剖乳头肌。将制备物固定在组织浴中，并用台氏液灌流。用薄膜技术在玻璃上制备了Ag/AgCl电极的线性和二维阵列。将具有多达24个电极（间隔50、90或180微米）的透明传感器定位在靠近制备物表面的位置，并使用定制设计的三维显微操纵器。细胞外信号由24通道数据采集系统同时记录，每通道采样率为200 kHz，幅度分辨率为12位，最大数据长度为3 MB。电极阵列和底层制备的数字化视频图像用于识别记录位点的位置。一个基于UNIX的计算机系统与定制设计的数据采集和数据库程序被用来控制仪器和管理实验数据。该技术给出了具有优异的信噪比（高达65 dB）的信号，并且允许以高分辨率（在5微秒内，50微米）精确评估局部激活的时间和位置。我们描述了几个细胞的区域内的兴奋的传播，并发现了大量的分散的传导速度。心跳到心跳的激活模式的比较表明，相对较小的变化，在传播的兴奋（几微秒）。
To study the spread of excitation in ventricular heart preparations we have designed a fast, high-resolution recording and mapping system. Papillary muscles were dissected from the isolated guinea pig hearts. The preparation was fixed in a tissue bath and superfused with Tyrode solution. Linear and two-dimensional arrays of Ag/AgCl electrodes were made on glass with a thin-film technique. The transparent sensors with up to 24 electrodes (spaced 50, 90, or 180 microns apart) were positioned close to the surface of the preparation with a custom-designed three-dimensional micromanipulator. Extracellular signals were simultaneously recorded by a 24-channel data acquisition system with a 200 kHz per channel sample rate, with 12-bit amplitude resolution and a maximum data length of up to 3 MB. Digitized video images of the electrode array and the underlaying preparation were used to identify the locations of the recording sites. A UNIX-based computer system with a custom-designed data acquisition and database program was used to control the instruments and to manage the experimental data. This technique gave signals with excellent signal-to-noise ratios (up to 65 dB) and permitted accurate evaluation of the time and the site of the local activation with high resolution (to within 5 microseconds, 50 microns). We describe the spread of excitation within the area of a few cells and found a substantial dispersion of conduction velocities. Beat-to-beat comparison of activation patterns showed relatively small variations in the spread of excitation (a few microseconds).