Platinum Nanoparticle Size and Density Impacts Purine Electrochemistry with Fast-Scan Cyclic Voltammetry.
Platinum Nanoparticle Size and Density Impacts Purine Electrochemistry with Fast-Scan Cyclic Voltammetry.
复制标题
铂纳米颗粒的尺寸和密度通过快速扫描循环伏安法影响嘌呤电化学。
DOI：
10.1149/1945-7111/ac65bc
复制
发表时间：
2022
影响因子：
3.9
通讯作者：
Ross,AshleyE
中科院分区：
文献类型：
--
作者：
Keller,AlexandraL;Quarin,StevenM;Strobbia,Pietro;Ross,AshleyE
我们通过快速扫描循环伏安法 (FSCV) 证明了碳纤维微电极上铂纳米粒子 (PtNP) 的密度和形状直接影响腺苷的检测。此前，我们证明金属纳米粒子修饰的碳显着改善了基于腺嘌呤的嘌呤检测；然而，尚未研究颗粒的尺寸和形状如何影响电化学检测。关于表面拓扑和形态如何影响检测的电化学研究对于设计超灵敏电极和扩展电极-分析物相互作用的基础知识是必要的。为了改变表面上 PtNP 的密度和形状，我们改变了 K 2 PtCl 6 的浓度和电沉积时间。我们表明，增加 K 2 PtCl 6 的浓度会增加 PtNP 的密度，而增加电沉积时间会影响密度和尺寸。这些变化会影响吸附行为，从而影响灵敏度。根据这些结果，确定最佳电沉积程序为 1.0 mg ml− 1 K 2 PtCl 6 沉积 45 秒，这导致腺苷检测平均增加 3.5±0.3 倍。有趣的是，增加 PtNP 的大小和密度会对多巴胺检测产生负面影响。总的来说，这项工作提供了关于电极表面腺苷和多巴胺相互作用之间差异的基本见解。
We demonstrate the density and shape of platinum nanoparticles (PtNP) on carbon-fiber microelectrodes with fast-scan cyclic voltammetry (FSCV) directly impacts detection of adenosine. Previously, we showed that metal nanoparticle-modified carbon significantly improves adenine-based purine detection; however, how the size and shape of the particles impact electrochemical detection was not investigated. Electrochemical investigations of how the surface topology and morphology impacts detection is necessary for designing ultrasensitive electrodes and for expanding fundamental knowledge of electrode-analyte interactions. To change the density and shape of the PtNP's on the surface, we varied the concentration of K 2 PtCl 6 and electrodeposition time. We show that increasing the concentration of K 2 PtCl 6 increases the density of PtNP's while increasing the electrodeposition time impacts both the density and size. These changes manipulate the adsorption behavior which impacts sensitivity. Based on these results, an optimal electrodeposition procedure was determined to be 1.0 mg ml− 1 of K 2 PtCl 6 deposited for 45 s and this results in an average increase in adenosine detection by 3.5±0.3-fold. Interestingly, increasing the size and density of PtNPs negatively impacts dopamine detection. Overall, this work provides fundamental insights into the differences between adenosine and dopamine interaction at electrode surfaces.