The Involvement of Jasmonic Acid, Ethylene, and Salicylic Acid in the Signaling Pathway of Clonostachys rosea-Induced Resistance to Gray Mold Disease in Tomato.
The Involvement of Jasmonic Acid, Ethylene, and Salicylic Acid in the Signaling Pathway of Clonostachys rosea-Induced Resistance to Gray Mold Disease in Tomato.
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DOI：
10.1094/phyto-01-19-0025-r
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发表时间：
2019-06
期刊：
影响因子：
3.2
通讯作者：
Qiuying Wang;Xiuling Chen;Xinfeng Chai;Dongqi Xue;Wei-wei Zheng;Yuying Shi;Aoxue Wang
中科院分区：
文献类型：
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作者：
Qiuying Wang;Xiuling Chen;Xinfeng Chai;Dongqi Xue;Wei-wei Zheng;Yuying Shi;Aoxue Wang
番茄灰霉病是由番茄灰霉病引起的一种严重威胁世界番茄生产的病害。华支睾吸虫已被成功地用作包括灰霉病在内的不同植物病原菌的生防剂。为了了解玫瑰花诱导番茄灰霉病抗性的信号转导途径，研究了玫瑰花对番茄灰霉病抗性的影响，以及4种不同激素缺陷型番茄灰霉病抗性相关基因(丝裂原活化蛋白激酶、WRKY、Lexyl2和ATPA)以及三种防御酶(苯丙氨酸解氨酶、多酚氧化酶和过氧化氢酶)、第二信使(一氧化氮、过氧化氢和超氧阴离子自由基)活性的变化。和赤霉素)番茄突变体进行了研究。结果表明，玫瑰花对番茄灰霉病菌的菌丝生长和孢子萌发有明显的抑制作用。此外，还能降低番茄灰霉病的发病率，诱导番茄叶片中PAL和PPO水平升高，CAT活性降低。此外，还能提高NO、H_2O_2、O_2-水平以及WRKY、MAPK、ATPA和Lexyl2的基因表达水平。4个激素缺乏突变体的灰霉病发病率均高于相应的野生型番茄。在所有这些激素缺乏的番茄突变体中，JA在调节不同信号分子方面的作用最为显著。另外的研究表明，JA上调了Lexyl2、MAPK和WRKY的表达，但下调了ATPA的表达。此外，茉莉酸还提高了PAL、PPO和CAT的活性以及NO和H_2O_2的产生。SA下调CAT和PAL的表达，而ET上调PAL但下调CAT的表达。本研究对了解玫瑰花对番茄灰霉病的调控途径和生防机理具有重要意义。
Tomato gray mold disease caused by Botrytis cinerea is a serious disease that threatens tomato production around the world. Clonostachys rosea has been used successfully as a biocontrol agent against divergent plant pathogens, including B. cinerea. To understand the signal transduction pathway of C. rosea-induced resistance to tomato gray mold disease, the effects of C. rosea on gray mold tomato leaves along with changes in the activities of three defense enzymes (phenylalanine ammonialyase [PAL], polyphenol oxidase [PPO], and catalase [CAT]), second messengers (nitric oxide [NO], hydrogen peroxide [H2O2], and superoxide anion radical [O2-]), and stress-related genes (mitogen-activated protein kinase [MAPK], WRKY, Lexyl2, and atpA) in four different hormone-deficient (jasmonic acid [JA], ethylene [ET], salicylic acid [SA], and gibberellin) tomato mutants were investigated. The results revealed that C. rosea significantly inhibited the growth of mycelia and spore germination of B. cinerea. Furthermore, it reduced the incidence of gray mold disease, induced higher levels of PAL and PPO, and induced lower levels of CAT activities in tomato leaves. Moreover, it also increased NO, H2O2, and O2- levels and the gene expression levels of WRKY, MAPK, atpA, and Lexyl2. The incidence of gray mold disease in four hormone-deficient mutants was higher than that in the corresponding wild-type tomato plants. Among all of these hormone-deficient tomato mutants, JA had the most significant effect in regulating the different signal molecules. Additional study suggested that JA upregulated the expression of Lexyl2, MAPK, and WRKY but downregulated atpA. Furthermore, JA also enhanced the activity of PAL, PPO, and CAT and the production of NO and H2O2. SA downregulated CAT and PAL, whereas ET upregulated PAL but downregulated CAT. This study is of significance in understanding the regulatory pathways and biocontrol mechanism of C. rosea against B. cinerea.