Tracing native and inoculated Rhizophagus irregularis in three potato cultivars (Charlotte, Nicola and Bintje) grown under field conditions
Tracing native and inoculated Rhizophagus irregularis in three potato cultivars (Charlotte, Nicola and Bintje) grown under field conditions
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DOI：
10.1016/j.apsoil.2017.03.007
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发表时间：
2017-07-01
影响因子：
4.8
通讯作者：
Cranenbrouck, Sylvie
中科院分区：
文献类型：
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作者：
Buysens, Catherine;Alaux, Pierre-Louis;Cranenbrouck, Sylvie
农作物接种丛枝菌根真菌（AMF）是一种很有前途的选择，以提高植物产量。然而，在大多数情况下，接种的菌株不能追溯到外地和他们的贡献，从本地AMF的根定殖。因此，没有明确的迹象表明生长促进与接种的分离株严格相关。在此，在比利时的田间条件下，将Rhizophagus irregularis MUCL 41833接种在三个马铃薯品种（Bintje、Nicola、夏洛特）上。田间试验前，将接种物包埋于海藻酸钠微球中，在温室条件下用剂量-反应关系估算菌根感染潜力（MIP）。线粒体大亚基（mtLSU）的变化。irregularis MUCL 41833进行单倍型设计。irregularis单倍型）和种特异性（天然或接种R.不规则）标记。通过实时定量PCR确定的标记检测的幅度与马铃薯品种的定殖阶段有关。Bintje在温室条件下生长。在田间条件下，R.与天然R相比，irregularis MUCL 41833在边缘数量的植物中以非常低的水平（在10（-6）和10（-7）ng/ng总DNA之间）被检测到。在三个品种的所有植株中检测到较高水平（在10（-4）和10（-6）ng/ng总DNA之间）的irregularis菌株。这表明，由于环境条件、与土著AMF的竞争或接种物放置不当，接种菌株在植物中几乎不存在。这是证实了没有生长促进和接种与非接种的马铃薯植物之间的根定殖的差异。这项研究验证了mtLSU标记检测/跟踪和量化AMF接种物作为在田间条件下生长的植物中的天然菌株，并进一步支持相同田间条件下的马铃薯品种在根定殖中的差异。(C)2017爱思唯尔B. V.保留所有权利。
Crop inoculation with arbuscular mycorrhizal fungi (AMF) is a promising option to increase plant yield. However, in most cases, the inoculated strains could not be traced in the field and their contribution to root colonization separated from native AMF. Therefore, there is no clear indication that growth promotion is strictly related to the inoculated isolates. Here, Rhizophagus irregularis MUCL 41833 was inoculated on three potato cultivars (Bintje, Nicola, Charlotte) under field conditions in Belgium. Inoculum was encapsulated into alginate beads and mycorrhizal infective potential (MIP) estimated with a dose-response relationship under greenhouse conditions before field experiment. Mitochondrial Large SubUnit (mtLSU) of inoculated R. irregularis MUCL 41833 was characterized to design haplotype(inoculated R. irregularis haplotype) and species-specific (native or inoculated R. irregularis) markers. The magnitude of detection of the markers, determined by Real-Time quantitative PCR, was linked to the stage of colonization of potato cv. Bintje grown under greenhouse conditions. Under field conditions, the inoculant R. irregularis MUCL 41833 was detected at a very low level (between 10(-6) and 10(-7) ng/ng total DNA) in a marginal number of plants, in contrast to native R. irregularis strains that were detected at higher levels (between 10(-4) and 10(-6) ng/ng total DNA) in all plants of the three cultivars. This suggested that the inoculated strain was almost absent in the plants due either to the environmental conditions, competition with indigenous AMF or inadequate placement of inoculum. This was corroborated by the absence of growth promotion and differences in root colonization between inoculated versus non inoculated potato plants. This study validated the mtLSU markers to detect/trace and quantify AMF inoculants as native strains in plants grown under field conditions and further supported that potato cultivars in the same field conditions differed in root colonization. (C) 2017 Elsevier B.V. All rights reserved.