Measurement of rat brain tumor kinetics using an intravascular MR contrast agent and DCE-MRI nested model selection.
Measurement of rat brain tumor kinetics using an intravascular MR contrast agent and DCE-MRI nested model selection.
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DOI：
10.1002/jmri.24469
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发表时间：
2014-11
期刊：
影响因子：
--
通讯作者：
Ewing JR
中科院分区：
文献类型：
--
作者：
Chwang WB;Jain R;Bagher-Ebadian H;Nejad-Davarani SP;Iskander AS;VanSlooten A;Schultz L;Arbab AS;Ewing JR
在大鼠胶质瘤模型中使用动态对比增强磁共振成像（DCE-MRI）和巢式模型选择（NMS），比较两种不同造影剂（CA）-钆磷维司（可逆结合人血清白蛋白）和钆喷酸葡胺（不结合）的药代动力学参数vp、Ktranss和ve的估计值。使用钆磷维塞和钆喷替酸盐对9只脑内接种9 L胶质肉瘤细胞的Fisher 344大鼠进行DCE-MRI研究。使用NMS估计参数vp、Ktranss和ve。与钆喷替酸盐相比，使用钆磷维塞的Ktranss估计值的平均值不同（钆磷维塞0.025 ± 0.008 min−1 vs.钆喷替酸盐0.046 ± 0.011 min−1; P = 0.0039）。尽管存在这种差异，但两种Ktranss估计值的组内相关系数（ICC）显示出近乎完美的线性相关性（ICC = 0.8479，Pearson r）。其他估计，（钆磷维司22.7 ± 4.7% vs.钆喷酸盐23.6 ± 5.6%; P = 0.4258）和vp（钆磷维司1.5 ± 0.5% vs.钆喷酸1.6 ± 0.4%; P = 0.25），两种CA之间的平均值无差异，两种CA之间的ve几乎完全一致（ICC = 0.8798），vp基本一致（ICC = 0.7981）。使用钆磷维塞和钆喷替酸盐的Ktranss估计值具有统计学差异，而使用两种CA的ve和vp相似。NMS使用DCE-MRI对药代动力学参数进行了稳健的估计，显示出作为肿瘤生理学和微环境重要指标的前景。
Using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in a rat glioma model, and nested model selection (NMS), to compare estimates of the pharmacokinetic parameters vp, Ktrans, and ve for two different contrast agents (CAs)—gadofosveset, which reversibly binds to human serum albumin, and gadopentetate dimeglumine, which does not. DCE-MRI studies were performed on nine Fisher 344 rats inoculated intracerebrally with 9L gliosarcoma cells using both gadofosveset and gadopentetate. The parameters vp, Ktrans, and ve were estimated using NMS. Ktrans estimates using gadofosveset, compared to gadopentetate, differed in their means (gadofosveset 0.025 ± 0.008 min−1 vs. gadopentetate 0.046 ± 0.011 min−1; P = 0.0039). This difference notwithstanding, the intraclass correlation coefficient (ICC) for the two estimates of Ktrans showed nearly perfect linear dependence (ICC = 0.8479 by Pearson’s r). Other estimates, ve (gadofosveset 22.7 ± 4.7% vs. gadopentetate 23.6 ± 5.6%; P = 0.4258) and vp (gadofosveset 1.5 ± 0.5% vs. gadopentetate 1.6 ± 0.4%; P = 0.25), were not different in their means between the two CAs, and there was almost perfect agreement for ve (ICC = 0.8798) and substantial agreement for vp (ICC = 0.7981) between the two CAs. Estimates of Ktrans were statistically different using gadofosveset and gadopentetate, whereas ve and vp were similar with two CAs. NMS produced robust estimates of pharmacokinetic parameters using DCE-MRI that show promise as important measures of tumor physiology and microenvironment.