Organic textile dye improves the visual assessment of the bait-lamina test
Organic textile dye improves the visual assessment of the bait-lamina test
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DOI：
10.1016/j.apsoil.2014.05.008
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发表时间：
2014-10-01
影响因子：
4.8
通讯作者：
Steinauer, Katja
中科院分区：
文献类型：
--
作者：
Eisenhauer, Nico;Wirsch, Daniela;Steinauer, Katja
大规模生态毒理学研究和协调分布式实验需要快速的生态系统评估。诱饵层条纹通常被用作评估分解者活动的标准化方法，但通常难以区分诱饵基质和土壤。在本研究中，我们的目的是确定一种染色方法，提高分解率的视觉评估的精度，同时具有可忽略不计的副作用。在含酸性或碱性土壤的微型环境中，对5种不同染料（食用染料、复活节草染料、有机纺织染料、油墨和墙漆）与对照基质进行了比较，并引入了两种线虫（假丝酵母菌和Sinella coeca）。有机纺织染料显示出最高的视觉评价精度，并且对分解速率、土壤微生物活性（生物量和呼吸）或线虫密度没有检测到的副作用。我们建议使用有机纺织染料来改善诱饵层试验，因为它精度高，易于制备。(C) 2014 Elsevier B.V.版权所有
Rapid ecosystem assessments are needed for large-scale ecotoxicological studies and coordinated distributed experiments. Bait-lamina stripes are commonly used as a standardized method to assess decomposer activity, but it is often difficult to distinguish bait substrate from soil. In the present study our aim was to identify a dyeing method that improves the precision of visual assessment of decomposition rates, while having negligible side effects. We compared five different dyes (food dye, Easter Grass, organic textile dye, ink, and wall paint) with control substrate in microcosms containing either acidic or alkaline soil with two introduced Collembola species (Folsomia candida and Sinella coeca). Organic textile dye showed the highest precision of visual assessment, and had no detectable side effects on decomposition rates, soil microbial activity (biomass and respiration), or Collembola densities. We recommend using organic textile dye to improve the bait-lamina test due to the high precision and the ease of preparation. (C) 2014 Elsevier B.V. All rights reserved.