Myristoylation of gag proteins of HIV-1 plays an important role in virus assembly.
Myristoylation of gag proteins of HIV-1 plays an important role in virus assembly.
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DOI：
10.1089/aid.1990.6.721
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发表时间：
1990-06
影响因子：
1.5
通讯作者：
R. Pal;Marvin S. Reitz;Erwin Tschachler;Robert C. Gallo;M. Sarngadharan;F. D. Veronese
中科院分区：
文献类型：
--
作者：
R. Pal;Marvin S. Reitz;Erwin Tschachler;Robert C. Gallo;M. Sarngadharan;F. D. Veronese
HIV-1的gag蛋白通过在氨基末端甘氨酸残基上添加肉豆蔻酸来修饰。使用定点诱变来构建HIV-1的突变体，其中该甘氨酸残基被改变为丙氨酸。转染到cos-1细胞后，突变基因组指导HIV-1蛋白的完整补体的合成，但p17和p17-含有多聚蛋白没有豆蔻酰化。用突变体DNA转染的细胞不释放任何病毒颗粒，并且通过电子显微镜看不到病毒核心。此外，来自这些转染细胞的上清液不能感染CEM细胞。gp 120在用突变体DNA转染的细胞表面上的表达和功能不受影响，因为这些细胞形成的合胞体在大小和数量上与用野生型DNA获得的合胞体相当。
The gag proteins of HIV-1 are modified by the addition of myristic acid to the amino terminal glycine residue. Site-directed mutagenesis was used to construct a mutant of HIV-1 in which this glycine residue was changed to an alanine. Upon transfection into cos-1 cells, the mutant genome directed the synthesis of the full complement of HIV-1 proteins, but p17 and p17-containing polyproteins were not myristoylated. The cells transfected with the mutant DNA did not release any virus particles and no viral cores were visible by electron microscopy. Furthermore, supernatant from these transfected cells failed to infect CEM cells. The expression and function of gp120 on the surface of cells transfected with the mutant DNA was unaffected as these cells formed syncytia comparable in both size and number to the ones obtained with wild-type DNA.