Liposomes and blood cells: A flow cytometric study
Liposomes and blood cells: A flow cytometric study
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DOI：
10.1081/bio-120025410
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发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Gyongyossy-Issa, M
中科院分区：
文献类型：
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作者：
Constantinescu, I;Levin, E;Gyongyossy-Issa, M
为了阐明脂质体与血细胞的相互作用，本研究检查了在缓冲液或血浆中、或在全血中、或在小鼠体内存在纯化的人血细胞的情况下一系列组合物的脂质体的行为。将用亲水性荧光染料、羧基荧光素（CF）或膜螯合R18或FITC标记的磷脂标记的脂质体与血细胞混合，并通过流式细胞术监测血细胞群中荧光染料的外观。无论组合物，有或没有聚（乙二醇），所有类型的脂质体被发现与红细胞，白细胞和血小板，在体外和体内迅速和剂量依赖性相互作用。这在存在和不存在血浆蛋白和功能性酶级联的情况下同样发生，表明初显相互作用是调理独立的，并且与脂质体-血细胞融合一致。
To clarify the interactions of liposomes with blood cells, this study examined the behaviour of liposomes of a range of compositions in the presence of purified human blood cells in buffer or plasma; or in whole blood, or in mice in vivo. Liposomes, labeled with the hydrophilic fluorochrome, carboxy fluorescein (CF), or with membrane-sequestering R 18 or FITC-labeled phospholipids, were mixed with blood cells and the appearance of the fluorochromes in the blood cell population was monitored by flow cytometry. Irrespective of composition, with or without poly(ethylene glycol), all types of liposomes were found to interact rapidly and dose-dependently with red cells, leukocytes and platelets, both in vitro and in vivo. This took place equally in the presence and the absence of plasma proteins and functional enzyme cascades, suggesting that the prime facie interaction is opsonization-independent and is consistent with liposome-blood cell fusion.