Relationship of endotoxin to tumor necrosis factor-α and interleukin-1β in children with otitis media with effusion
Relationship of endotoxin to tumor necrosis factor-α and interleukin-1β in children with otitis media with effusion
复制标题
DOI：
10.1177/000348949810700106
复制
发表时间：
1998-01-01
影响因子：
1.4
通讯作者：
DeMaria, TF
中科院分区：
文献类型：
--
作者：
Willett, DN;Rezaee, RP;DeMaria, TF
对41例慢性渗出性中耳炎患儿的65例中耳积液和配对血清进行了内毒素、肿瘤坏死因子-α（TNF-α）和白细胞介素-1 β（IL-1 β）检测，以确定内毒素浓度和这些细胞因子浓度之间是否存在相关性。通过显色鲎试剂试验测定内毒素浓度，通过定量酶联免疫吸附试验测定细胞因子浓度。40%的渗出液具有可检测水平的内毒素，平均浓度为2.9 +/- 7.8内毒素单位/毫克总蛋白。TNF-α的平均浓度为1.24 +/- 3.1 pg/mg总蛋白，IL-1 β的平均浓度为18.79 pg/mg总蛋白。内毒素浓度与TNF-α（r =.89）和IL-1 β（r =.72）之间存在强的统计学显著相关性。这些数据表明，内毒素可能通过刺激中耳中TNF-α和IL-1 β的持续产生而导致慢性渗出性中耳炎的发病。
Sixty-five middle ear effusions and paired sera from 41 children with chronic otitis media with effusion were assayed for endotoxin and for tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in order to establish whether a correlation exists between the concentrations of endotoxin and of these cytokines. Endotoxin concentration was determined by means of a chromogenic limulus amebocyte lysate assay, and the cytokine concentration by means of a quantitative enzyme-linked immunosorbent assay. Forty percent of the effusions had detectable levels of endotoxin, with a mean concentration of 2.9 +/- 7.8 endotoxin units per milligram of total protein. The mean concentration of TNF-alpha was 1.24 +/- 3.1 pg/mg total protein, and that of IL-1 beta was 18.79 pg/mg total protein. A strong, statistically significant correlation exists between the concentrations of endotoxin and TNF-alpha (r =.89) and IL-1 beta (r =.72). The data indicate that endotoxin may contribute to the pathogenesis of chronic otitis media with effusion by stimulating the sustained production of TNF-alpha and IL-1 beta in the middle ear.