Catalytic promiscuity in the biosynthesis of cyclic peptide secondary metabolites in planktonic marine cyanobacteria
Catalytic promiscuity in the biosynthesis of cyclic peptide secondary metabolites in planktonic marine cyanobacteria
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DOI：
10.1073/pnas.0913677107
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发表时间：
2010-06-08
影响因子：
11.1
通讯作者：
van der Donk, Wilfred A.
中科院分区：
文献类型：
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作者：
Li, Bo;Sher, Daniel;van der Donk, Wilfred A.
我们对细菌次级代谢产物的理解主要是通过对共生体、病原体和土壤细菌等附着种类的研究形成的。在这里，我们表明，单细胞的，atmosphonic海洋蓝细菌原绿球藻，它进行了相当大的比例在海洋中的光合作用，产生许多环状，羊毛硫氨酸含肽（lantipeptides）的菌株。值得注意的是，在原绿球藻MIT 9313一个单一的混杂酶转化多达29个不同的线性核糖体合成的肽到一个多环，构象约束的产品具有高度多样的环拓扑结构库。编码这个系统的基因在不同的海洋中被发现，在加拉帕戈斯的高盐泻湖中有一个热点，这表明它们在特定的栖息地和/或社区中发挥着作用。产生结构多样性的非常有效的途径使这些蓝藻能够产生与模式产藻细菌一样多的次级代谢产物，但基因组要小得多。
Our understanding of secondary metabolite production in bacteria has been shaped primarily by studies of attached varieties such as symbionts, pathogens, and soil bacteria. Here we show that a strain of the single-celled, planktonic marine cyanobacterium Prochlorococcus-which conducts a sizable fraction of photosynthesis in the oceans-produces many cyclic, lanthionine-containing peptides (lantipeptides). Remarkably, in Prochlorococcus MIT9313 a single promiscuous enzyme transforms up to 29 different linear ribosomally synthesized peptides into a library of polycyclic, conformationally constrained products with highly diverse ring topologies. Genes encoding this system are found in variable abundances across the oceans-with a hot spot in a Galapagos hypersaline lagoon-suggesting they play a habitat-and/or community-specific role. The extraordinarily efficient pathway for generating structural diversity enables these cyanobacteria to produce as many secondary metabolites as model antibiotic-producing bacteria, but with much smaller genomes.