Differentiation of human pluripotent stem cells into pancreatic duct-like organoids.
Differentiation of human pluripotent stem cells into pancreatic duct-like organoids.
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DOI：
10.1016/j.xpro.2021.100913
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发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Kleger A
中科院分区：
文献类型：
--
作者：
Breunig M;Merkle J;Melzer MK;Heller S;Seufferlein T;Meier M;Hohwieler M;Kleger A
人类发育过程和病理表现的重演需要在胚胎发生和疾病期间获得特定的细胞类型和前体阶段。在这里，我们描述了一种可扩展的体外分化方案，以引导人类多能干细胞逐步进入胰管样类器官。该方案模拟胰管发育，并成功用于模拟胰腺导管腺癌的发病和进展;该方法适用于多种下游应用。然而，该协议是成本和时间密集型的。有关本方案使用和执行的完整详细信息，请参阅。从hPSC生成胰管样类器官（PDLO）的分步方案3D Matrigel类器官培养物的回收、加工和分裂体外分化模拟胰管发育成功用于模拟胰腺癌的发作和进展人类发育过程和病理表现的重演需要在胚胎发生和疾病期间获得特定的细胞类型和前体阶段。在这里，我们描述了一种可扩展的体外分化方案，以引导人类多能干细胞逐步进入胰管样类器官。该方案模拟胰管发育，并成功用于模拟胰腺导管腺癌的发病和进展;该方法适用于多种下游应用。然而，该协议是成本和时间密集型的。
The recapitulation of human developmental processes and pathological manifestations requires access to specific cell types and precursor stages during embryogenesis and disease. Here, we describe a scalable in vitro differentiation protocol to guide human pluripotent stem cells stepwise into pancreatic duct-like organoids. The protocol mimics pancreatic duct development and was successfully used to model the onset and progression of pancreatic ductal adenocarcinoma; the approach is suitable for multiple downstream applications. However, the protocol is cost- and time-intensive. For complete details on the use and execution of this protocol, please refer to. Step-by step protocol to generate pancreatic duct-like organoids (PDLOs) from hPSCs Recovery, processing, and splitting of the 3D Matrigel organoid culture In vitro differentiation mimics pancreatic duct development Successfully used to model the onset and progression of pancreatic cancer The recapitulation of human developmental processes and pathological manifestations requires access to specific cell types and precursor stages during embryogenesis and disease. Here, we describe a scalable in vitro differentiation protocol to guide human pluripotent stem cells stepwise into pancreatic duct-like organoids. The protocol mimics pancreatic duct development and was successfully used to model the onset and progression of pancreatic ductal adenocarcinoma; the approach is suitable for multiple downstream applications. However, the protocol is cost- and time-intensive.
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影响因子：
5.9
作者：
Nostro, M. Cristina;Sarangi, Farida;Yang, Chaoxing;Holland, Andrew;Elefanty, Andrew G.;Stanley, Edouard G.;Greiner, Dale L.;Keller, Gordon
通讯作者：
Keller, Gordon
糖蛋白2是人类胰腺祖细胞的特定细胞表面标记。
影响因子：
16.6
作者：
Cogger KF;Sinha A;Sarangi F;McGaugh EC;Saunders D;Dorrell C;Mejia-Guerrero S;Aghazadeh Y;Rourke JL;Screaton RA;Grompe M;Streeter PR;Powers AC;Brissova M;Kislinger T;Nostro MC
通讯作者：
Nostro MC
影响因子：
7.7
作者：
Ramond, Cyrille;Glaser, Nicolas;Scharfmannt, Raphael
通讯作者：
Scharfmannt, Raphael
影响因子：
23.9
作者：
Breunig M;Merkle J;Wagner M;Melzer MK;Barth TFE;Engleitner T;Krumm J;Wiedenmann S;Cohrs CM;Perkhofer L;Jain G;Krüger J;Hermann PC;Schmid M;Madácsy T;Varga Á;Griger J;Azoitei N;Müller M;Wessely O;Robey PG;Heller S;Dantes Z;Reichert M;Günes C;Bolenz C;Kuhn F;Maléth J;Speier S;Liebau S;Sipos B;Kuster B;Seufferlein T;Rad R;Meier M;Hohwieler M;Kleger A
通讯作者：
Kleger A