Structures of fecapentaenes, the mutagens of bacterial origin isolated from human feces.
Structures of fecapentaenes, the mutagens of bacterial origin isolated from human feces.
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Fecapentaenes 的结构，这是从人类粪便中分离出来的细菌来源的诱变剂。
DOI：
10.1021/bi00271a001
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
J. Krepinsky
中科院分区：
文献类型：
--
作者：
I. Gupta;J. Baptista;W. Bruce;C. Che;R. Furrer;J. Gingerich;A. A. Grey;L. Marai;P. Yates;J. Krepinsky
方法所有操作均在暗室中进行（仅允许台灯远离工作台；玻璃器皿为红色或用铝箔包裹），通常在氮气或氩气气氛中进行。色谱法：(1)在Florisil柱上进行萃取色谱法。将粪便混合物（1.8 kg，参见材料）置于在 C6H6 中制备的 Florisil (1.5-kg) 柱顶部，并用 C6H6 (3 L)、CH2Cl2 (2.5 L)、20% Me2CO 的 CH2Cl2 溶液 (4 L) 和 Me2CO (2 L) 洗涤。诱变物质通常在倒数第二个级分中洗脱，通过 TLC（含 10% MeOH 的 CHC13）监测，通过长波紫外灯（Mineralight）将其可视化为绿色荧光点。向含有致突变物质的级分中添加0.01%抗氧化剂BHT（丁基羟基甲苯，Sigma）；这个数量的BHT
MethodsAll operations were done in a darkened room(only desk lamps allowed far away from benches; the glassware was either red or wrapped in an aluminum foil), usually inN2 or Ar atmosphere. Chromatography:(1) Extractive Chromatography on a Florisil Column. Feces mix (1.8 kg, cf. Materials) was placed on top of a Florisil (1.5-kg) column prepared in C6H6 and washed with C6H6 (3 L), CH2C12 (2.5 L), 20% Me2CO inCH2C12 (4 L), and Me2CO (2 L). Mutagenic substances were usually eluted in the penultimate fraction as monitored by TLC in 10% MeOH in CHC13 visualized by long-wave UV lamps (Mineralight) as a green fluorescent spot. To the fractions containing mutagenic substances was added 0.01% antioxidant BHT (butylated hydroxytoluene, Sigma); this amount of BHT
DOI：
10.1016/0027-5107(80)90122-0
发表时间：
1980
期刊：
Mutation research
影响因子：
--
作者：
Reddy,BS;Sharma,C;Darby,L;Laakso,K;Wynder,EL
通讯作者：
Wynder,EL