Transcranial Recording of Electrophysiological Neural Activity in the Rodent Brain in vivo Using Functional Photoacoustic Imaging of Near-Infrared Voltage-Sensitive Dye
Transcranial Recording of Electrophysiological Neural Activity in the Rodent Brain in vivo Using Functional Photoacoustic Imaging of Near-Infrared Voltage-Sensitive Dye
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DOI：
10.3389/fnins.2019.00579
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发表时间：
2019-08-09
影响因子：
4.3
通讯作者：
Boctor, Emad M.
中科院分区：
文献类型：
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作者：
Kang, Jeeun;Zhang, Haichong K.;Boctor, Emad M.
微创监测电生理神经活动的实时-这使得量化的神经功能，而不需要有创开颅手术和较长的时间常数的功能磁共振成像和PET-提出了一个非常具有挑战性的，但重要的任务，神经成像。在本文中，我们提出了在体内功能性PA（fPA）成像的化学惊厥大鼠癫痫发作模型与完整的头皮使用荧光猝灭为基础的花青电压敏感染料（VSD），其特征在于由脂质囊泡模型模仿不同水平的膜电位变化。该框架还涉及使用通过血脑屏障（BBB）递送的近红外VSD，通过腺苷受体信号传导的药理学调节打开。我们的标准化时间-频率分析显示，在亚毫米空间分辨率下，癫痫发作组的VSD反应与对照组的VSD反应有显著区别。脑电图（EEG）记录证实了化学惊厥引起的脑活动的严重程度和频率的变化。研究结果表明，近红外fPA VSD成像是一个有前途的工具，在体内记录大脑活动，通过完整的头皮，这将铺平道路，其未来的翻译在真实的时间人脑成像。
Minimally-invasive monitoring of electrophysiological neural activities in real-time-that enables quantification of neural functions without a need for invasive craniotomy and the longer time constants of fMRI and PET-presents a very challenging yet significant task for neuroimaging. In this paper, we present in vivo functional PA (fPA) imaging of chemoconvulsant rat seizure model with intact scalp using a fluorescence quenching-based cyanine voltage-sensitive dye (VSD) characterized by a lipid vesicle model mimicking different levels of membrane potential variation. The framework also involves use of a near-infrared VSD delivered through the blood-brain barrier (BBB), opened by pharmacological modulation of adenosine receptor signaling. Our normalized time-frequency analysis presented in vivo VSD response in the seizure group significantly distinguishable from those of the control groups at sub-mm spatial resolution. Electroencephalogram (EEG) recording confirmed the changes of severity and frequency of brain activities, induced by chemoconvulsant seizures of the rat brain. The findings demonstrate that the near-infrared fPA VSD imaging is a promising tool for in vivo recording of brain activities through intact scalp, which would pave a way to its future translation in real time human brain imaging.