Differential expression of IGFBP-5 and two human ESTs in thyroid glands with goiter, adenoma and papillary or follicular carcinomas
Differential expression of IGFBP-5 and two human ESTs in thyroid glands with goiter, adenoma and papillary or follicular carcinomas
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DOI：
10.1016/s0304-3835(02)00679-1
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发表时间：
2003-03-10
期刊：
影响因子：
9.7
通讯作者：
Reis, LFL
中科院分区：
文献类型：
--
作者：
Stolf, BS;Carvalho, AF;Reis, LFL
在这里，我们描述了三个人类基因的识别与甲状腺疾病的表达改变。其中之一对应于胰岛素样生长因子结合蛋白5（IGFBP 5），其已经被描述为在其他癌症中过表达，并且首次被鉴定为在甲状腺肿瘤中过表达。其他基因，命名为44和199，是功能未知的EST，分别定位在人类第七和第四染色体上。我们通过RT-PCR测定了这些基因在10例无病甲状腺、10例甲状腺肿、9例乳头状癌、10例腺瘤和7例滤泡状癌中的表达水平，并对观察到的差异进行了统计学分析。IGFBP 5和基因44在乳头状癌中的表达显著高于正常和甲状腺肿。与正常甲状腺组织相比，基因44和199在滤泡癌和腺瘤中差异表达。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Here, we describe the identification of three human genes with altered expression in thyroid diseases. One of them corresponds to insulin-like growth factor binding protein 5 (IGFBP5), which has already been described as over expressed in other cancers and, for the first time, is identified as overexpressed in thyroid tumors. The other genes, named 44 and 199, are ESTs with yet unknown function and were mapped on human chromosomes seven and four, respectively. We determined by RT-PCR the expression level of these genes in ten samples of disease-free thyroid, ten of goiter, nine of papillary carcinoma, ten of adenoma and seven of follicular carcinoma and the significance of observed differences was statistically determined. IGFBP5 and gene 44 were significantly overexpressed in papillary carcinoma when compared to normal and goiter. Genes 44 and 199 were differentially expressed in follicular carcinoma and adenoma when compared to normal thyroid tissue. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.