ACTIVATED, HTLV-1-SPECIFIC CYTOTOXIC LYMPHOCYTES-T ARE FOUND IN HEALTHY SEROPOSITIVES AS WELL AS IN PATIENTS WITH TROPICAL SPASTIC PARAPARESIS
ACTIVATED, HTLV-1-SPECIFIC CYTOTOXIC LYMPHOCYTES-T ARE FOUND IN HEALTHY SEROPOSITIVES AS WELL AS IN PATIENTS WITH TROPICAL SPASTIC PARAPARESIS
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DOI：
10.1016/0042-6822(92)90517-s
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发表时间：
1992-06-01
期刊：
影响因子：
3.7
通讯作者：
BANGHAM, CRM
中科院分区：
文献类型：
--
作者：
PARKER, CE;DAENKE, S;BANGHAM, CRM
在人类T细胞淋巴瘤/白血病病毒（HTLV-1）感染的热带痉挛性截瘫（TSP）患者中，循环中存在活化的HTLV-1特异性细胞毒性T淋巴细胞（CTL），脊髓病变中淋巴细胞浸润富含CD8+ T细胞。这些观察结果提示病毒特异性CTL在TSP发病机制中的作用。我们检测了HTLV-1血清阳性的8名受试者外周血淋巴细胞中新分离的CD8+ T细胞的抗HTLV-1细胞毒活性。5例TSP患者中有4例有循环活化的抗tax CTL。然而，没有TSP的3名血清阳性受试者中有2名也激活了抗tax CTL。这些观察结果表明，这种活化的CTL并不局限于TSP患者，并对其在该病发病机制中的意义提出了一些不确定性。在两名TSP受试者的CD8+ T细胞培养中，我们在没有外源抗原刺激的情况下检测到具有其他HTLV-1特异性的CTL。已经在肽水平上定位了一个位于Tax中间区域的CTL表位和一个位于Pol C端的CTL表位，并确定了限制它们识别的HLA 1类分子。
In human T cell lymphoma/leukemia virus (HTLV-1)-infected people with tropical spastic paraparesis (TSP), there are activated HTLV-1-specific cytotoxic T lymphocytes (CTL) in the circulation and lymphocytic infiltrates in spinal cord lesions that are rich in CD8+ T cells. These observations suggest a role for virus-specific CTL in the pathogenesis of TSP. We have examined the anti-HTLV-1 cytotoxic activity of freshly isolated CD8+ T cells from peripheral blood lymphocytes of eight subjects seropositive for HTLV-1. Four of five subjects with TSP had circulating activated anti-Tax CTL. However, two of three seropositive subjects without TSP also had activated anti-Tax CTL. These observations show that such activated CTL are not confined to patients with TSP and raise some uncertainty about their significance in the pathogenesis of the disease. In cultures of CD8+ T cells from two TSP subjects, we detected CTL with other HTLV-1 specificities, without exogenous antigenic stimulation. A CTL epitope in the middle region of Tax and one in the C terminus of Pol have been mapped at the peptide level and the HLA Class 1 molecules restricting their recognition have been defined.