Comprehensive Study of the Flow Control Strategy in a Wirelessly Charged Centrifugal Microfluidic Platform with Two Rotation Axes
Comprehensive Study of the Flow Control Strategy in a Wirelessly Charged Centrifugal Microfluidic Platform with Two Rotation Axes
复制标题
无线充电两轴离心微流控平台流量控制策略综合研究
DOI：
10.1021/acs.analchem.7b02080
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发表时间：
2017
影响因子：
7.4
通讯作者：
Cheng Jing
中科院分区：
文献类型：
--
作者：
Zhu Yunzeng;Chen Yiqi;Meng Xiangrui;Wang Jing;Lu Ying;Xu Youchun;Cheng Jing
离心微流控技术已广泛应用于核酸、蛋白质和小分子的分析中。然而，单向流体推进的固有特性限制了这些流控芯片的灵活性。提供额外的自由度以允许液体的不受约束和可逆的泵送是解决该限制的有效策略。本研究构建了一个具有两个旋转轴的无线充电离心式微流控平台，并首次对该平台的两自由度流动控制策略进行了全面研究。感应耦合线圈安装在平台上，以实现无线电力传输到旋转阶段。微型伺服电机安装在载物台的两侧，以在载物台旋转期间根据需要改变装置围绕第二旋转轴的取向。对该平台上的液体定向输送、阀门控制、计量、混合等基本操作进行了全面的研究和实现。最后，基于本文提出的策略，设计了一种同时测定水样中六价铬和甲醛的芯片，并进行了测试，证明了该平台在现场环境监测、食品安全检测等生命科学领域的应用潜力。
Centrifugal microfluidics has been widely applied in the sample-in–answer-out systems for the analyses of nucleic acids, proteins, and small molecules. However, the inherent characteristic of unidirectional fluid propulsion limits the flexibility of these fluidic chips. Providing an extra degree of freedom to allow the unconstrained and reversible pumping of liquid is an effective strategy to address this limitation. In this study, a wirelessly charged centrifugal microfluidic platform with two rotation axes has been constructed and the flow control strategy in such platform with two degrees of freedom was comprehensively studied for the first time. Inductively coupled coils are installed on the platform to achieve wireless power transfer to the spinning stage. A micro servo motor is mounted on both sides of the stage to alter the orientation of the device around a secondary rotation axis on demand during stage rotation. The basic liquid operations on this platform, including directional transport of liquid, valving, metering, and mixing, are comprehensively studied and realized. Finally, a chip for the simultaneous determination of hexavalent chromium [Cr(VI)] and methanal in water samples is designed and tested based on the strategy presented in this paper, demonstrating the potential use of this platform for on-site environmental monitoring, food safety testing, and other life science applications.