Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter
Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter
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DOI：
10.1126/science.1150559
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发表时间：
2008-01-25
期刊：
影响因子：
56.9
通讯作者：
McNally, James G.
中科院分区：
文献类型：
--
作者：
Karpova, Tatiana S.;Kim, Min J.;McNally, James G.
对于基因调控，一些转录激活因子以快（类似于 1 分钟）或慢（类似于 15 至 90 分钟）周期周期性地与启动子结合。目前尚不清楚快循环是否发生在天然启动子上，并且任一循环在转录中的功能仍不清楚。我们报告说，快速和慢速循环可以同时发生在内源酵母启动子上，并且该系统中的慢速循环反映了可及启动子比例的振荡，而不是稳定结合的转录激活子的招募和释放。这一观察结果与信使 RNA (mRNA) 产生的单细胞测量相结合，认为快速循环启动转录，而慢速循环调节产生的 mRNA 的数量。这些发现反驳了慢循环启动转录的流行观点。
For gene regulation, some transcriptional activators bind periodically to promoters with either a fast (similar to 1 minute) or a slow (similar to 15 to 90 minutes) cycle. It is uncertain whether the fast cycle occurs on natural promoters, and the function of either cycle in transcription remains unclear. We report that fast and slow cycling can occur simultaneously on an endogenous yeast promoter and that slow cycling in this system reflects an oscillation in the fraction of accessible promoters rather than the recruitment and release of stably bound transcriptional activators. This observation, combined with single- cell measurements of messenger RNA ( mRNA) production, argues that fast cycling initiates transcription and that slow cycling regulates the quantity of mRNA produced. These findings counter the prevailing view that slow cycling initiates transcription.