Recent advances in RNA sequence analysis.
Recent advances in RNA sequence analysis.
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DOI：
10.3410/b2-64
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发表时间：
2010-08-19
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影响因子：
--
通讯作者：
Salzberg, Steven L
中科院分区：
文献类型：
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作者：
Salzberg, Steven L
最新的高通量DNA测序技术现在可以大规模应用于捕获细胞中完整的mRNA转录本，使用称为RNA-seq的技术。虽然RNA-seq只有2岁，但它已经迅速席卷了基因组学领域，现在它被用于分析从细菌到灵长类动物的生物体的转录组。测序的深度使研究人员能够量化基因的表达水平，发现真核生物中的替代亚型，甚至描述细菌基因组的操纵子结构。
The latest high-throughput DNA sequencing technology can now be applied on a large scale to capture the complete set of mRNA transcripts in a cell, using a technique called RNA-seq. Although RNA-seq is only 2 years old, it has rapidly swept through the field of genomics, and it is now being used to analyze the transcriptomes of organisms ranging from bacteria to primates. The depth of sequencing allows researchers to quantify the level of expression of genes, to discover alternative isoforms in eukaryotic species, and even to characterize the operon structure of bacterial genomes.