Efferent neural projections of angiotensin receptor (AT1) expressing neurones in the hypothalamic paraventricular nucleus of the rat
Efferent neural projections of angiotensin receptor (AT1) expressing neurones in the hypothalamic paraventricular nucleus of the rat
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DOI：
10.1046/j.1365-2826.2001.00597.x
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发表时间：
2001-02-01
影响因子：
3.2
通讯作者：
McKinley, MJ
中科院分区：
文献类型：
--
作者：
Oldfield, BJ;Davern, PJ;McKinley, MJ
血管紧张素II在下丘脑室旁核（PVN）内起作用，帮助介导一些自主神经和内分泌反应。关于在PVN内表现出血管紧张素II型1受体的特定神经元细胞群的证据很少，并且不存在与核中特定的传出神经元群相关的证据。在本实验中，逆行转运的神经元示踪剂与免疫组织化学结合使用，使用一种特性良好的针对at(1)受体羧基端十肽序列的多克隆抗体，以确定它是否优先分布在PVN内的不同输出群体中。AT(1)受体与PVN中将轴突投射到脊髓、髓内背外侧或腹外侧的神经元无关，但与核前旁细胞分裂中将轴突投射到正中隆起的神经元密切共存。这类神经元常含有促肾上腺皮质激素释放因子。这些发现强调了PVN中的血管紧张素II和AT(1)受体可能在应激反应的介导中起作用。
Angiotensin II acts within the hypothalamic paraventricular nucleus (PVN) to help mediate a number of autonomic and endocrine responses. Evidence is sparse in regard to the particular neuronal cell groups that exhibit angiotensin II type 1 receptors within the PVN, and does not exist in relation to specified efferent neuronal populations in the nucleus. In the present experiments, retrogradely transported neuronal tracers were utilized in conjunction with immunohistochemistry using a well characterized polyclonal antibody raised against a decapeptide sequence at the carboxy terminus of the AT(1) receptor, to determine whether it is preferentially distributed amongst different efferent populations within the PVN. The AT(1) receptor is not associated with neurones in the PVN that project axons to the spinal cord, dorsomedial or ventrolateral medulla but coexists strongly with neurones in the anterior parvocellular division of the nucleus which direct axons to the median eminence. Such neurones often contain corticotropin releasing factor. These findings highlight the role that angiotensin II and AT(1) receptors in the PVN may play in the mediation of responses to stress.