ACTIVITY-DEPENDENT REGULATION OF GLUTAMIC-ACID DECARBOXYLASE IN THE RAT BARREL CORTEX - EFFECTS OF NEONATAL VERSUS ADULT SENSORY DEPRIVATION
ACTIVITY-DEPENDENT REGULATION OF GLUTAMIC-ACID DECARBOXYLASE IN THE RAT BARREL CORTEX - EFFECTS OF NEONATAL VERSUS ADULT SENSORY DEPRIVATION
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DOI：
10.1002/cne.903070204
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发表时间：
1991-05-08
影响因子：
2.5
通讯作者：
LAND, PW
中科院分区：
文献类型：
--
作者：
AKHTAR, ND;LAND, PW
用免疫细胞化学技术研究了触觉剥夺对大鼠躯体感觉桶皮质含谷氨酸脱羧酶(GAD)神经元的影响。正常大鼠各板层均有GAD免疫反应阳性神经元和斑点，其中密集的GAD免疫反应斑点集中在IV板层的毛管上。成年大鼠剪去胡须后，与毛发修剪相对应的毛桶中GAD免疫反应活性可逆性降低。GAD染色的强度也可逆地改变在被剥夺的桶两侧的非剥夺桶的颗粒上板层中。这表明桶状皮质中的GAD水平通常会随着感觉输入的变化而波动。相比之下，从出生起就修剪胡须的动物在被剥夺和未被剥夺的桶中都有正常的GAD染色。此外，如果让刚出生时被剥夺的动物的胡须生长到正常长度，并在成年后重新修剪，GAD染色不会受到影响。因此，早期的实际剥夺扰乱了允许在感觉体验改变后调节皮质神经元中的递质酶水平的机制。
Immunocytochemical techniques were used to study the effects of tactual deprivation on glutamic acid decarboxylase (GAD) containing neurons in rat somatosensory barrel cortex. In normal rats GAD immunoreactive neurons and puncta are present in all laminae, with dense patches of GAD immunoreactive puncta centered on the barrels in lamina IV. Trimming whiskers of adult rats leads to a reversible decrease of GAD immunoreactivity in barrels corresponding to trimmed hairs. Intensity of GAD staining also is reversibly altered in supragranular laminae of nondeprived barrel columns flanked by deprived barrels. This indicates that GAD levels in the barrel cortex ordinarily fluctuate with changes in sensory input. By contrast, animals whose whiskers are trimmed from birth have normal GAD staining in both deprived and nondeprived barrels. Moreover, if trimmed whiskers of neonatally deprived animals are allowed to grow to normal lengths and are retrimmed later in adulthood GAD staining is not affected. Thus early tactual deprivation disrupts mechanisms that permit modulation of transmitter enzyme levels in cortical neurons following changes in sensory experience.