Spatial regulation of the actin cytoskeleton by HSF-1 during aging.
Spatial regulation of the actin cytoskeleton by HSF-1 during aging.
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DOI：
10.1091/mbc.e18-06-0362
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发表时间：
2018-10-15
影响因子：
3.3
通讯作者：
Dillin A
中科院分区：
文献类型：
--
作者：
Higuchi-Sanabria R;Paul JW 3rd;Durieux J;Benitez C;Frankino PA;Tronnes SU;Garcia G;Daniele JR;Monshietehadi S;Dillin A
有许多研究表明，肌动蛋白细胞骨架与年龄相关的下降，这已经被衰老生物学领域的常识所采用。然而，在衰老的多细胞生物中，这种现象的直接鉴定尚未进行。在这里，我们以组织特异性的方式表达LifeAct::mRuby，以询问细胞骨架组织作为年龄的函数。我们首次在秀丽隐杆线虫中发现，肌动蛋白细胞骨架的组织和形态在老年肌肉、肠道和皮下组织中恶化。此外，在衰老过程中，hsf-1对于调节细胞骨架的完整性至关重要，因此，hsf-1的下调会导致肌动蛋白的过早衰老，而其过表达会保护肌肉、肠道和皮下组织中肌动蛋白细胞骨架的完整性。最后，仅在神经元中过表达hsf-1就足以保护非神经元细胞的细胞骨架完整性。
There are many studies suggesting an age-associated decline in the actin cytoskeleton, and this has been adopted as common knowledge in the field of aging biology. However, a direct identification of this phenomenon in aging multicellular organisms has not been performed. Here, we express LifeAct::mRuby in a tissue-specific manner to interrogate cytoskeletal organization as a function of age. We show for the first time in Caenorhabditis elegans that the organization and morphology of the actin cytoskeleton deteriorate at advanced age in the muscles, intestine, and hypodermis. Moreover, hsf-1 is essential for regulating cytoskeletal integrity during aging, so that knockdown of hsf-1 results in premature aging of actin and its overexpression protects actin cytoskeletal integrity in the muscles, the intestine, and the hypodermis. Finally, hsf-1 overexpression in neurons alone is sufficient to protect cytoskeletal integrity in nonneuronal cells.